Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Nasal immunization with the C-terminal domain of BclA3 induced specific IgG production and attenuated disease symptoms in mice infected with Clostridioides difficile spores

Ana Raquel Maia, Rodrigo Reyes-Ramírez, Marjorie Pizarro-Guajardo, Anella Saggese, Ezio Ricca, Loredana Baccigalupi, Daniel Paredes-Sabja
doi: https://doi.org/10.1101/2020.05.31.125435
Ana Raquel Maia
1Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 26, 80126 Napoli, Italy; (A.R.M.); (A.S.); (E.R.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: raqueldiasmaia@outlook.com ornellasaggese2010@libero.it ericca@unina.it
Rodrigo Reyes-Ramírez
2Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Avenida Republica 330, 8370186 Santiago, Chile; (R.R.-R.); (M.P.-G.)
3Millennium Nucleus in the Biology of Intestinal Microbiota, Avenida Republica 330, 8370186 Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rodrigoreyesr1992@gmail.com marjorie.pizarrog@gmail.com
Marjorie Pizarro-Guajardo
2Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Avenida Republica 330, 8370186 Santiago, Chile; (R.R.-R.); (M.P.-G.)
3Millennium Nucleus in the Biology of Intestinal Microbiota, Avenida Republica 330, 8370186 Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rodrigoreyesr1992@gmail.com marjorie.pizarrog@gmail.com
Anella Saggese
1Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 26, 80126 Napoli, Italy; (A.R.M.); (A.S.); (E.R.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: raqueldiasmaia@outlook.com ornellasaggese2010@libero.it ericca@unina.it
Ezio Ricca
1Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia 26, 80126 Napoli, Italy; (A.R.M.); (A.S.); (E.R.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: raqueldiasmaia@outlook.com ornellasaggese2010@libero.it ericca@unina.it
Loredana Baccigalupi
4Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via Pansini 5, 80131 Napoli, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lorbacci@unina.it daniel.paredes.sabja@gmail.com
Daniel Paredes-Sabja
2Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Avenida Republica 330, 8370186 Santiago, Chile; (R.R.-R.); (M.P.-G.)
3Millennium Nucleus in the Biology of Intestinal Microbiota, Avenida Republica 330, 8370186 Santiago, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lorbacci@unina.it daniel.paredes.sabja@gmail.com rodrigoreyesr1992@gmail.com marjorie.pizarrog@gmail.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Clostridioides difficile is a Gram-positive, spore-forming bacterium that causes a severe intestinal infection. Spores of this pathogen enter in the human body through the oral route, interact with intestinal epithelial cells and persist in the gut. Once germinated, the vegetative cells colonize the intestine and produce toxins that enhance a strong immune response that perpetuate the disease. Therefore, spores are major players of the infection and ideal targets of new therapeutic treatments. In this context, spore surface proteins of C. difficile, are potential antigens for the development of vaccines targeting C. difficile spores. Here we report that the C-terminal domain of the spore surface protein BclA3, BclA3CTD, was identified as an antigenic epitope, over-produced in Escherichia coli and tested as an immunogen in mice. To increase antigen stability and efficiency, BclA3CTD was also exposed on the surface of B. subtilis spores, a well-established mucosal vaccine delivery system. In the experimental conditions used in this study, free BclA3CTD induced antibody production in mice and attenuated some CDI symptoms after a challenge with the pathogen, while the spore-displayed antigen resulted less effective. Although dose regimen and immunization route need to be optimized, our results suggest BclA3CTD as a potentially effective antigen to develop a new vaccination strategy targeting C. difficile spores.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted May 31, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Nasal immunization with the C-terminal domain of BclA3 induced specific IgG production and attenuated disease symptoms in mice infected with Clostridioides difficile spores
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Nasal immunization with the C-terminal domain of BclA3 induced specific IgG production and attenuated disease symptoms in mice infected with Clostridioides difficile spores
Ana Raquel Maia, Rodrigo Reyes-Ramírez, Marjorie Pizarro-Guajardo, Anella Saggese, Ezio Ricca, Loredana Baccigalupi, Daniel Paredes-Sabja
bioRxiv 2020.05.31.125435; doi: https://doi.org/10.1101/2020.05.31.125435
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Nasal immunization with the C-terminal domain of BclA3 induced specific IgG production and attenuated disease symptoms in mice infected with Clostridioides difficile spores
Ana Raquel Maia, Rodrigo Reyes-Ramírez, Marjorie Pizarro-Guajardo, Anella Saggese, Ezio Ricca, Loredana Baccigalupi, Daniel Paredes-Sabja
bioRxiv 2020.05.31.125435; doi: https://doi.org/10.1101/2020.05.31.125435

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9624)
  • Bioengineering (7118)
  • Bioinformatics (24929)
  • Biophysics (12659)
  • Cancer Biology (9985)
  • Cell Biology (14395)
  • Clinical Trials (138)
  • Developmental Biology (7986)
  • Ecology (12141)
  • Epidemiology (2067)
  • Evolutionary Biology (16021)
  • Genetics (10947)
  • Genomics (14774)
  • Immunology (9897)
  • Microbiology (23730)
  • Molecular Biology (9502)
  • Neuroscience (51038)
  • Paleontology (370)
  • Pathology (1544)
  • Pharmacology and Toxicology (2690)
  • Physiology (4035)
  • Plant Biology (8687)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6453)
  • Zoology (1349)