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Comprehensive characterization of internal and cuticle surface microbiota of laboratory-reared F1 Anopheles albimanus originating from different sites

View ORCID ProfileNsa Dada, Ana Cristina Benedict, Francisco López, View ORCID ProfileJuan C. Lol, Mili Sheth, Nicole Dzuris, View ORCID ProfileNorma Padilla, View ORCID ProfileAudrey Lenhart
doi: https://doi.org/10.1101/2020.06.02.129619
Nsa Dada
aEntomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, United States Centers for Diseases Control and Prevention, Atlanta, GA, United States of America
dAmerican Society for Microbiology, Washington, DC, United States of America
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  • For correspondence: nsadada@yahoo.com
Ana Cristina Benedict
bGrupo de Biología y Control de Vectores, Centro de Estudios en Salud, Universidad del Valle de Guatemala, Guatemala
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Francisco López
bGrupo de Biología y Control de Vectores, Centro de Estudios en Salud, Universidad del Valle de Guatemala, Guatemala
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Juan C. Lol
bGrupo de Biología y Control de Vectores, Centro de Estudios en Salud, Universidad del Valle de Guatemala, Guatemala
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Mili Sheth
cBiotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging & Zoonotic Infectious Diseases, United States Centers for Disease Control and Prevention, Atlanta, GA, United States of America
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Nicole Dzuris
aEntomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, United States Centers for Diseases Control and Prevention, Atlanta, GA, United States of America
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Norma Padilla
bGrupo de Biología y Control de Vectores, Centro de Estudios en Salud, Universidad del Valle de Guatemala, Guatemala
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Audrey Lenhart
aEntomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, United States Centers for Diseases Control and Prevention, Atlanta, GA, United States of America
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Abstract

Background Research on mosquito-microbe interactions may lead to new tools for mosquito and mosquito-borne disease control. To date, such research has largely utilized laboratory-reared mosquitoes that typically lack the microbial diversity of wild populations. A logical progression in this area involves working under controlled settings using field-collected mosquitoes or, in most cases, their progeny. Thus, an understanding of how laboratory colonization affects the assemblage of mosquito microbiota would aid in advancing mosquito microbiome studies and their applications beyond laboratory settings.

Methods Using high throughput 16S rRNA amplicon sequencing, we characterized the internal and cuticle surface microbiota of F1 progeny of wild-caught adult Anopheles albimanus from four locations in Guatemala. A total of 132 late instar larvae and 135 2-5day old, non-blood-fed virgin adult females that were reared under identical laboratory conditions, were pooled (3 individuals/pool) and analyzed.

Results Results showed geographical heterogeneity in both F1 larval internal (p=0.001; pseudo-F = 9.53) and cuticle surface (p=0.001; pseudo-F = 8.51) microbiota, and only F1 adult cuticle surface (p=0.001; pseudo-F = 4.5) microbiota, with a more homogenous adult internal microbiota (p=0.12; pseudo-F = 1.6) across collection sites. Overall, ASVs assigned to Leucobacter, Thorsellia, Chryseobacterium and uncharacterized Enterobacteriaceae, dominated F1 larval internal microbiota, while Acidovorax, Paucibacter, and uncharacterized Comamonadaceae, dominated the larval cuticle surface. F1 adults comprised a less diverse microbiota compared to larvae, with ASVs assigned to the genus Asaia dominating both internal and cuticle surface microbiota, and constituting at least 70% of taxa in each microbial niche.

Conclusions These results suggest that location-specific heterogeneity in filed mosquito microbiota can be transferred to F1 progeny under normal laboratory conditions, but this may not last beyond the F1 larval stage without adjustments to maintain field-derived microbiota. Our findings provide the first comprehensive characterization of laboratory-colonized F1 An. albimanus progeny from field-derived mothers. This provides a background for studying how parentage and environmental conditions differentially or concomitantly affect mosquito microbiome composition, and how this can be exploited in advancing mosquito microbiome studies and their applications beyond laboratory settings.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA512122

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 18, 2021.
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Comprehensive characterization of internal and cuticle surface microbiota of laboratory-reared F1 Anopheles albimanus originating from different sites
Nsa Dada, Ana Cristina Benedict, Francisco López, Juan C. Lol, Mili Sheth, Nicole Dzuris, Norma Padilla, Audrey Lenhart
bioRxiv 2020.06.02.129619; doi: https://doi.org/10.1101/2020.06.02.129619
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Comprehensive characterization of internal and cuticle surface microbiota of laboratory-reared F1 Anopheles albimanus originating from different sites
Nsa Dada, Ana Cristina Benedict, Francisco López, Juan C. Lol, Mili Sheth, Nicole Dzuris, Norma Padilla, Audrey Lenhart
bioRxiv 2020.06.02.129619; doi: https://doi.org/10.1101/2020.06.02.129619

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