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Purification of age-distinct insulin secretory granules through antigen restriction using the CLIP-tag

View ORCID ProfileMartin Neukam, Katharina Ganß, View ORCID ProfileJovana Vasiljević, View ORCID ProfileJohannes Broichhagen, View ORCID ProfileKai Johnsson, View ORCID ProfileThomas Kurth, View ORCID ProfileMichele Solimena
doi: https://doi.org/10.1101/2020.06.03.103770
Martin Neukam
1Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany
3German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
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  • ORCID record for Martin Neukam
Katharina Ganß
1Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany
3German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
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Jovana Vasiljević
1Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany
3German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
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  • ORCID record for Jovana Vasiljević
Johannes Broichhagen
4Max-Planck-Institute for Medical Research, Department of Chemical Biology, Heidelberg, Germany
5Forschungsinstitut für Molekulare Pharmakologie, Department of Chemical Biology, Berlin, Germany
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Kai Johnsson
4Max-Planck-Institute for Medical Research, Department of Chemical Biology, Heidelberg, Germany
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  • ORCID record for Kai Johnsson
Thomas Kurth
6TU Dresden, Center for Molecular and Cellular Bioengineering (CMCB), Technology Platform, Dresden, Germany
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Michele Solimena
1Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany
3German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
7Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
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  • ORCID record for Michele Solimena
  • For correspondence: Michele.Solimena@uniklinikum-dresden.de
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Article Information

doi 
https://doi.org/10.1101/2020.06.03.103770
History 
  • June 3, 2020.

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  • You are currently viewing Version 1 of this article (June 3, 2020 - 16:06).
  • View Version 2, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Martin Neukam1,2,3,
  2. Katharina Ganß1,2,3,
  3. Jovana Vasiljević1,2,3,
  4. Johannes Broichhagen4,5,
  5. Kai Johnsson4,
  6. Thomas Kurth6 and
  7. Michele Solimena1,2,3,7,#
  1. 1Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany
  2. 2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany
  3. 3German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
  4. 4Max-Planck-Institute for Medical Research, Department of Chemical Biology, Heidelberg, Germany
  5. 5Forschungsinstitut für Molekulare Pharmakologie, Department of Chemical Biology, Berlin, Germany
  6. 6TU Dresden, Center for Molecular and Cellular Bioengineering (CMCB), Technology Platform, Dresden, Germany
  7. 7Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
  1. ↵#Corresponding author. Tel.: +49-351-796-366-11, e-mail: Michele.Solimena{at}uniklinikum-dresden.de
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Posted June 03, 2020.
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Purification of age-distinct insulin secretory granules through antigen restriction using the CLIP-tag
Martin Neukam, Katharina Ganß, Jovana Vasiljević, Johannes Broichhagen, Kai Johnsson, Thomas Kurth, Michele Solimena
bioRxiv 2020.06.03.103770; doi: https://doi.org/10.1101/2020.06.03.103770
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Purification of age-distinct insulin secretory granules through antigen restriction using the CLIP-tag
Martin Neukam, Katharina Ganß, Jovana Vasiljević, Johannes Broichhagen, Kai Johnsson, Thomas Kurth, Michele Solimena
bioRxiv 2020.06.03.103770; doi: https://doi.org/10.1101/2020.06.03.103770

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