Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Identification of rare transient somatic cell states induced by injury and required for whole-body regeneration

View ORCID ProfileBlair W. Benham-Pyle, Carolyn E. Brewster, Aubrey M. Kent, Frederick G. Mann Jr., Shiyuan Chen, Allison R. Scott, View ORCID ProfileAndrew C. Box, View ORCID ProfileAlejandro Sánchez Alvarado
doi: https://doi.org/10.1101/2020.06.04.132753
Blair W. Benham-Pyle
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Blair W. Benham-Pyle
  • For correspondence: asa@stowers.org bbp@stowers.org
Carolyn E. Brewster
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aubrey M. Kent
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Frederick G. Mann Jr.
1Stowers Institute for Medical Research, Kansas City, MO, USA
2Howard Hughes Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shiyuan Chen
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Allison R. Scott
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew C. Box
1Stowers Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Andrew C. Box
Alejandro Sánchez Alvarado
1Stowers Institute for Medical Research, Kansas City, MO, USA
2Howard Hughes Institute for Medical Research, Kansas City, MO, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alejandro Sánchez Alvarado
  • For correspondence: asa@stowers.org bbp@stowers.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Regeneration requires functional coordination of stem cells, their progeny, and differentiated cells. Past studies have focused on regulation of stem cell identity and proliferation near to the wound-site, but less is known about contributions made by differentiated cells distant to the injury. Here, we present a comprehensive atlas of whole-body regeneration over time and identify rare, transient, somatic cell states induced by injury and required for regeneration. To characterize amputation-specific signaling across a whole animal, 299,998 single-cell transcriptomes were captured from planarian tissue fragments competent and incompetent to regenerate. Amputation-specific cell states were rare, non-uniformly distributed across tissues, and particularly enriched in muscle (mesoderm), epidermis (ectoderm), and intestine (endoderm). Moreover, RNAi-mediated knockdown of genes up-regulated in amputation-specific cell states drastically reduced regenerative capacity. These results identify novel cell states and molecules required for whole-body regeneration and indicate that regenerative capacity requires transcriptional plasticity in a rare subset of differentiated cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • We noticed that some gene labels above UMAPs are missing in Figure 5. This revision addresses the formating error.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted June 06, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Identification of rare transient somatic cell states induced by injury and required for whole-body regeneration
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Identification of rare transient somatic cell states induced by injury and required for whole-body regeneration
Blair W. Benham-Pyle, Carolyn E. Brewster, Aubrey M. Kent, Frederick G. Mann Jr., Shiyuan Chen, Allison R. Scott, Andrew C. Box, Alejandro Sánchez Alvarado
bioRxiv 2020.06.04.132753; doi: https://doi.org/10.1101/2020.06.04.132753
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Identification of rare transient somatic cell states induced by injury and required for whole-body regeneration
Blair W. Benham-Pyle, Carolyn E. Brewster, Aubrey M. Kent, Frederick G. Mann Jr., Shiyuan Chen, Allison R. Scott, Andrew C. Box, Alejandro Sánchez Alvarado
bioRxiv 2020.06.04.132753; doi: https://doi.org/10.1101/2020.06.04.132753

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4246)
  • Biochemistry (9176)
  • Bioengineering (6807)
  • Bioinformatics (24069)
  • Biophysics (12161)
  • Cancer Biology (9568)
  • Cell Biology (13847)
  • Clinical Trials (138)
  • Developmental Biology (7662)
  • Ecology (11739)
  • Epidemiology (2066)
  • Evolutionary Biology (15547)
  • Genetics (10673)
  • Genomics (14366)
  • Immunology (9517)
  • Microbiology (22916)
  • Molecular Biology (9135)
  • Neuroscience (49170)
  • Paleontology (358)
  • Pathology (1488)
  • Pharmacology and Toxicology (2584)
  • Physiology (3851)
  • Plant Biology (8353)
  • Scientific Communication and Education (1473)
  • Synthetic Biology (2302)
  • Systems Biology (6207)
  • Zoology (1304)