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Woeseiales transcriptional response in Arctic fjord surface sediment

View ORCID ProfileJoy Buongiorno, Katie Sipes, Kenneth Wasmund, Alexander Loy, Karen G. Lloyd
doi: https://doi.org/10.1101/2020.06.04.134015
Joy Buongiorno
1Department of Microbiology, University of Tennessee, Knoxville, USA
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  • For correspondence: JBuongiorno@carnegiescience.edu
Katie Sipes
1Department of Microbiology, University of Tennessee, Knoxville, USA
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Kenneth Wasmund
2Division of Microbial Ecology, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria
3Austrian Polar Research Institute, Vienna, Austria
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Alexander Loy
2Division of Microbial Ecology, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria
3Austrian Polar Research Institute, Vienna, Austria
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Karen G. Lloyd
1Department of Microbiology, University of Tennessee, Knoxville, USA
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Abstract

Distinct lineages of Gammaproteobacteria clade Woeseiales are globally-distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted June 05, 2020.
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Woeseiales transcriptional response in Arctic fjord surface sediment
Joy Buongiorno, Katie Sipes, Kenneth Wasmund, Alexander Loy, Karen G. Lloyd
bioRxiv 2020.06.04.134015; doi: https://doi.org/10.1101/2020.06.04.134015
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Woeseiales transcriptional response in Arctic fjord surface sediment
Joy Buongiorno, Katie Sipes, Kenneth Wasmund, Alexander Loy, Karen G. Lloyd
bioRxiv 2020.06.04.134015; doi: https://doi.org/10.1101/2020.06.04.134015

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