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Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system

Ludovico Cantuti-Castelvetri, Ravi Ojha, Liliana D. Pedro, Minou Djannatian, Jonas Franz, Suvi Kuivanen, Katri Kallio, Tuğberk Kaya, Maria Anastasina, Teemu Smura, Lev Levanov, Leonora Szirovicza, Allan Tobi, Hannimari Kallio-Kokko, Pamela Österlund, Merja Joensuu, Frédéric A. Meunier, Sarah Butcher, Martin Sebastian Winkler, Brit Mollenhauer, Ari Helenius, Ozgun Gokce, Tambet Teesalu, Jussi Hepojoki, Olli Vapalahti, Christine Stadelmann, Giuseppe Balistreri, Mikael Simons
doi: https://doi.org/10.1101/2020.06.07.137802
Ludovico Cantuti-Castelvetri
1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
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Ravi Ojha
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
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Liliana D. Pedro
1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
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Minou Djannatian
1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
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Jonas Franz
4Department of Neuropathology, University Medical Center Göttingen, Göttingen, Germany
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Suvi Kuivanen
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
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Katri Kallio
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
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Tuğberk Kaya
1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
6Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, Munich, Germany
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Maria Anastasina
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
7Helsinki Institute of Life Sciences-Institute of Biotechnology, University of Helsinki, Finland
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Teemu Smura
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
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Lev Levanov
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
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Leonora Szirovicza
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
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Allan Tobi
8Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
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Hannimari Kallio-Kokko
9University of Helsinki and Helsinki University Hospital, Department of Virology, Helsinki, Finland
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Pamela Österlund
10Department of Health Security, Finnish Institute for Health and Welfare (THL), Helsinki, Finland
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Merja Joensuu
11Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
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Frédéric A. Meunier
11Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
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Sarah Butcher
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
7Helsinki Institute of Life Sciences-Institute of Biotechnology, University of Helsinki, Finland
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Martin Sebastian Winkler
12Department of Anesthesiology and Intensive Care Medicine, University Medical Center Göttingen, Göttingen, Germany
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Brit Mollenhauer
13Department of Neurology, University Medical Center Göttingen, Göttingen, Germany & Paracelsus-Elena-Klinik Kassel, Kassel, Germany
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Ari Helenius
14Institute of Biochemistry, ETH Zürich, Zürich, Switzerland
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Ozgun Gokce
6Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, Munich, Germany
19Munich Cluster of Systems Neurology (SyNergy), Munich, Germany
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Tambet Teesalu
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
15Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA
16Center for Nanomedicine and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA
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Jussi Hepojoki
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
17Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
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Olli Vapalahti
5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
9University of Helsinki and Helsinki University Hospital, Department of Virology, Helsinki, Finland
18Dept of Veterinary Biosciences, University of Helsinki, Helsinki, Finland
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Christine Stadelmann
4Department of Neuropathology, University Medical Center Göttingen, Göttingen, Germany
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Giuseppe Balistreri
3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
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  • For correspondence: giuseppe.balistreri@helsinki.fi mikael.simons@dzne.de
Mikael Simons
1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
19Munich Cluster of Systems Neurology (SyNergy), Munich, Germany
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  • For correspondence: giuseppe.balistreri@helsinki.fi mikael.simons@dzne.de
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Article Information

doi 
https://doi.org/10.1101/2020.06.07.137802
History 
  • July 15, 2020.

Article Versions

  • Version 1 (June 7, 2020 - 22:13).
  • Version 2 (June 10, 2020 - 11:54).
  • You are viewing Version 3, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Ludovico Cantuti-Castelvetri1,2,*,
  2. Ravi Ojha3,*,
  3. Liliana D. Pedro1,2,*,
  4. Minou Djannatian1,2,*,
  5. Jonas Franz4,*,
  6. Suvi Kuivanen5,*,
  7. Katri Kallio3,
  8. Tuğberk Kaya1,2,6,
  9. Maria Anastasina3,7,
  10. Teemu Smura5,
  11. Lev Levanov5,
  12. Leonora Szirovicza5,
  13. Allan Tobi8,
  14. Hannimari Kallio-Kokko9,
  15. Pamela Österlund10,
  16. Merja Joensuu11,
  17. Frédéric A. Meunier11,
  18. Sarah Butcher3,7,
  19. Martin Sebastian Winkler12,
  20. Brit Mollenhauer13,
  21. Ari Helenius14,
  22. Ozgun Gokce6,19,
  23. Tambet Teesalu3,15,16,
  24. Jussi Hepojoki5,17,
  25. Olli Vapalahti5,9,18,
  26. Christine Stadelmann4,
  27. Giuseppe Balistreri3,§ and
  28. Mikael Simons1,2,19,§
  1. 1Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany
  2. 2German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
  3. 3Faculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland
  4. 4Department of Neuropathology, University Medical Center Göttingen, Göttingen, Germany
  5. 5University of Helsinki, Medicum, Department of Virology, Helsinki, Finland
  6. 6Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, Munich, Germany
  7. 7Helsinki Institute of Life Sciences-Institute of Biotechnology, University of Helsinki, Finland
  8. 8Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
  9. 9University of Helsinki and Helsinki University Hospital, Department of Virology, Helsinki, Finland
  10. 10Department of Health Security, Finnish Institute for Health and Welfare (THL), Helsinki, Finland
  11. 11Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia
  12. 12Department of Anesthesiology and Intensive Care Medicine, University Medical Center Göttingen, Göttingen, Germany
  13. 13Department of Neurology, University Medical Center Göttingen, Göttingen, Germany & Paracelsus-Elena-Klinik Kassel, Kassel, Germany
  14. 14Institute of Biochemistry, ETH Zürich, Zürich, Switzerland
  15. 15Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA
  16. 16Center for Nanomedicine and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA
  17. 17Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
  18. 18Dept of Veterinary Biosciences, University of Helsinki, Helsinki, Finland
  19. 19Munich Cluster of Systems Neurology (SyNergy), Munich, Germany
  1. ↵§Correspondence: giuseppe.balistreri{at}helsinki.fi & mikael.simons{at}dzne.de
  1. ↵* Equal contributions

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Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system
Ludovico Cantuti-Castelvetri, Ravi Ojha, Liliana D. Pedro, Minou Djannatian, Jonas Franz, Suvi Kuivanen, Katri Kallio, Tuğberk Kaya, Maria Anastasina, Teemu Smura, Lev Levanov, Leonora Szirovicza, Allan Tobi, Hannimari Kallio-Kokko, Pamela Österlund, Merja Joensuu, Frédéric A. Meunier, Sarah Butcher, Martin Sebastian Winkler, Brit Mollenhauer, Ari Helenius, Ozgun Gokce, Tambet Teesalu, Jussi Hepojoki, Olli Vapalahti, Christine Stadelmann, Giuseppe Balistreri, Mikael Simons
bioRxiv 2020.06.07.137802; doi: https://doi.org/10.1101/2020.06.07.137802
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Neuropilin-1 facilitates SARS-CoV-2 cell entry and provides a possible pathway into the central nervous system
Ludovico Cantuti-Castelvetri, Ravi Ojha, Liliana D. Pedro, Minou Djannatian, Jonas Franz, Suvi Kuivanen, Katri Kallio, Tuğberk Kaya, Maria Anastasina, Teemu Smura, Lev Levanov, Leonora Szirovicza, Allan Tobi, Hannimari Kallio-Kokko, Pamela Österlund, Merja Joensuu, Frédéric A. Meunier, Sarah Butcher, Martin Sebastian Winkler, Brit Mollenhauer, Ari Helenius, Ozgun Gokce, Tambet Teesalu, Jussi Hepojoki, Olli Vapalahti, Christine Stadelmann, Giuseppe Balistreri, Mikael Simons
bioRxiv 2020.06.07.137802; doi: https://doi.org/10.1101/2020.06.07.137802

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