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Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike

Jérémie Prévost, Romain Gasser, Guillaume Beaudoin-Bussières, Jonathan Richard, Ralf Duerr, Annemarie Laumaea, Sai Priya Anand, Guillaume Goyette, Shilei Ding, Halima Medjahed, Antoine Lewin, Josée Perreault, Tony Tremblay, Gabrielle Gendron-Lepage, Nicolas Gauthier, Marc Carrier, Diane Marcoux, Alain Piché, Myriam Lavoie, Alexandre Benoit, Vilayvong Loungnarath, Gino Brochu, Marc Desforges, Pierre J. Talbot, Graham T. Gould Maule, Marceline Côté, Christian Therrien, Bouchra Serhir, Renée Bazin, Michel Roger, Andrés Finzi
doi: https://doi.org/10.1101/2020.06.08.140244
Jérémie Prévost
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Romain Gasser
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Guillaume Beaudoin-Bussières
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Jonathan Richard
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Ralf Duerr
3Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
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Annemarie Laumaea
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Sai Priya Anand
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
4Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada
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Guillaume Goyette
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
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Shilei Ding
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
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Halima Medjahed
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
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Antoine Lewin
5Héma-Québec, Affaires Médicales et Innovation, Québec, QC, G1V 5C3, Canada
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Josée Perreault
5Héma-Québec, Affaires Médicales et Innovation, Québec, QC, G1V 5C3, Canada
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Tony Tremblay
5Héma-Québec, Affaires Médicales et Innovation, Québec, QC, G1V 5C3, Canada
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Gabrielle Gendron-Lepage
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
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Nicolas Gauthier
6Hôpital Sacré-Cœur de Montréal, Montreal, QC H4J 1C5, Canada
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Marc Carrier
7Hôpital Cité-de-la-Santé, Laval, QC H7M 3L9, Canada
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Diane Marcoux
8Hôtel-Dieu de Lévis, Lévis, QC G6V 3Z1, Canada
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Alain Piché
9Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada
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Myriam Lavoie
10CIUSSS du Saguenay-Lac-Saint-Jean, Hôpital de Chicoutimi, Chicoutimi, QC G7H 5H6, Canada
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Alexandre Benoit
11Hôpital de Verdun, Montreal, QC H4G 2A3, Canada
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Vilayvong Loungnarath
12CHU de Québec, Hôpital Enfant-Jésus, Quebec, QC G1J 1Z4, Canada
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Gino Brochu
13CIUSSS de la Mauricie-et-du-Centre-du-Québec, Trois-Rivières, QC G9A 5C5, Canada
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Marc Desforges
14INRS-Institut Armand Frappier, Laval, QC H7V 1B7, Canada
15CHU Ste-Justine, Montreal, QC, H3T 1C5, Canada
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Pierre J. Talbot
14INRS-Institut Armand Frappier, Laval, QC H7V 1B7, Canada
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Graham T. Gould Maule
16Department of Biochemistry, Microbiology and Immunology, and Center for Infection, Immunity, and Inflammation, University of Ottawa, Ottawa, ON K1H 8M5, Canada
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Marceline Côté
16Department of Biochemistry, Microbiology and Immunology, and Center for Infection, Immunity, and Inflammation, University of Ottawa, Ottawa, ON K1H 8M5, Canada
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Christian Therrien
17Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue H9X 3R5, Canada
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Bouchra Serhir
17Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue H9X 3R5, Canada
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Renée Bazin
5Héma-Québec, Affaires Médicales et Innovation, Québec, QC, G1V 5C3, Canada
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Michel Roger
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
17Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue H9X 3R5, Canada
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Andrés Finzi
1Centre de Recherche du CHUM, QC H2X 0A9, Canada
2Departement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada
4Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada
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  • For correspondence: andres.finzi@umontreal.ca
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SUMMARY

The SARS-CoV-2 virus is responsible for the current worldwide coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds of thousands of deaths. The Spike glycoprotein of SARS-CoV-2 mediates viral entry and is the main target for neutralizing antibodies. Understanding the antibody response directed against SARS-CoV-2 is crucial for the development of vaccine, therapeutic and public health interventions. Here we performed a cross-sectional study on 98 SARS-CoV-2-infected individuals to evaluate humoral responses against the SARS-CoV-2 Spike. The vast majority of infected individuals elicited anti-Spike antibodies within 2 weeks after the onset of symptoms. The levels of receptor-binding domain (RBD)-specific IgG persisted overtime, while the levels of anti-RBD IgM decreased after symptoms resolution. Some of the elicited antibodies cross-reacted with other human coronaviruses in a genus-restrictive manner. While most of individuals developed neutralizing antibodies within the first two weeks of infection, the level of neutralizing activity was significantly decreased over time. Our results highlight the importance of studying the persistence of neutralizing activity upon natural SARS-CoV-2 infection.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike
Jérémie Prévost, Romain Gasser, Guillaume Beaudoin-Bussières, Jonathan Richard, Ralf Duerr, Annemarie Laumaea, Sai Priya Anand, Guillaume Goyette, Shilei Ding, Halima Medjahed, Antoine Lewin, Josée Perreault, Tony Tremblay, Gabrielle Gendron-Lepage, Nicolas Gauthier, Marc Carrier, Diane Marcoux, Alain Piché, Myriam Lavoie, Alexandre Benoit, Vilayvong Loungnarath, Gino Brochu, Marc Desforges, Pierre J. Talbot, Graham T. Gould Maule, Marceline Côté, Christian Therrien, Bouchra Serhir, Renée Bazin, Michel Roger, Andrés Finzi
bioRxiv 2020.06.08.140244; doi: https://doi.org/10.1101/2020.06.08.140244
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Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike
Jérémie Prévost, Romain Gasser, Guillaume Beaudoin-Bussières, Jonathan Richard, Ralf Duerr, Annemarie Laumaea, Sai Priya Anand, Guillaume Goyette, Shilei Ding, Halima Medjahed, Antoine Lewin, Josée Perreault, Tony Tremblay, Gabrielle Gendron-Lepage, Nicolas Gauthier, Marc Carrier, Diane Marcoux, Alain Piché, Myriam Lavoie, Alexandre Benoit, Vilayvong Loungnarath, Gino Brochu, Marc Desforges, Pierre J. Talbot, Graham T. Gould Maule, Marceline Côté, Christian Therrien, Bouchra Serhir, Renée Bazin, Michel Roger, Andrés Finzi
bioRxiv 2020.06.08.140244; doi: https://doi.org/10.1101/2020.06.08.140244

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