Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity Nanobody

Guillermo Valenzuela Nieto; Ronald Jara; Daniel Watterson; Naphak Modhiran; Alberto A Amarilla; Johanna Himelreichs; Alexander A. Khromykh; Constanza Salinas; Teresa Pinto; Yorka Cheuquemilla; Yago Margolles; Natalia López González del Rey; Zaray Miranda-Chacon; Alexei Cuevas; Anne Berking; Camila Deride; Sebastián González-Moraga; Héctor Mancilla; Daniel Maturana; Andreas Langer; Juan Pablo Toledo; Ananda Müller; Benjamín Uberti; Paola Krall; Pamela Ehrenfeld; Javier Blesa; Pedro Chana-Cuevas; German Rehren; David Schwefel; Luis Ángel Fernandez; Alejandro Rojas-Fernandez

doi:10.1101/2020.06.09.137935

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Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity Nanobody

Guillermo Valenzuela Nieto, Ronald Jara, Daniel Watterson, Naphak Modhiran, Alberto A Amarilla, Johanna Himelreichs, Alexander A. Khromykh, Constanza Salinas, Teresa Pinto, Yorka Cheuquemilla, Yago Margolles, Natalia López González del Rey, Zaray Miranda-Chacon, Alexei Cuevas, Anne Berking, Camila Deride, Sebastián González-Moraga, Héctor Mancilla, Daniel Maturana, Andreas Langer, Juan Pablo Toledo, Ananda Müller, Benjamín Uberti, Paola Krall, Pamela Ehrenfeld, Javier Blesa, Pedro Chana-Cuevas, German Rehren, David Schwefel, Luis Ángel Fernandez, Alejandro Rojas-Fernandez

doi: https://doi.org/10.1101/2020.06.09.137935

Guillermo Valenzuela Nieto

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Ronald Jara

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

2Institute of Biochemistry and Microbiology, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile

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Daniel Watterson

3School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, Australia

4The Australian Institute for Biotechnology and Nanotechnology, The University of Queensland, Brisbane, Australia

5Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Australia

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Naphak Modhiran

3School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, Australia

4The Australian Institute for Biotechnology and Nanotechnology, The University of Queensland, Brisbane, Australia

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Alberto A Amarilla

3School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, Australia

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Johanna Himelreichs

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Alexander A. Khromykh

3School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, Australia

5Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Australia

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Constanza Salinas

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Teresa Pinto

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Yorka Cheuquemilla

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

7Comprehensive Neuroscience Center AC (CINAC), HM Puerta del Sur, Madrid Mostoles Hospital, 28938 Madrid, Spain

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Yago Margolles

6Department of Microbial Biotechnology, National Biotechnology Center, Superior Council of Scientific Research, Madrid, Spain

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Natalia López González del Rey

7Comprehensive Neuroscience Center AC (CINAC), HM Puerta del Sur, Madrid Mostoles Hospital, 28938 Madrid, Spain

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Zaray Miranda-Chacon

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Alexei Cuevas

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Anne Berking

8Berking Biotechnology, Valdivia, Chile

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Camila Deride

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

11Institute of Veterinary Clinical Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile

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Sebastián González-Moraga

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Héctor Mancilla

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Daniel Maturana

9NanoTemper Technologies GmbH, Floessergasse 4, 81369 Munich, Germany

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Andreas Langer

9NanoTemper Technologies GmbH, Floessergasse 4, 81369 Munich, Germany

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Juan Pablo Toledo

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

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Ananda Müller

10Ross University School of Veterinary Medicine, Saint Kitts and Nevis, West Indies

11Institute of Veterinary Clinical Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile

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Benjamín Uberti

11Institute of Veterinary Clinical Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile

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Paola Krall

1Institute of Medicine, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile

12Department of Pediatrics and Children’s Surgery Oriente, Universidad de Chile

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Pamela Ehrenfeld

13Institute of Anatomy, Histology, and Pathology, Faculty of Medicine, Universidad Austral de Chile

17Center for Interdisciplinary Studies on the Nervous System, CISNE, Universidad Austral de Chile, Valdivia, Chile

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Javier Blesa

7Comprehensive Neuroscience Center AC (CINAC), HM Puerta del Sur, Madrid Mostoles Hospital, 28938 Madrid, Spain

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Pedro Chana-Cuevas

14CETRAM & Faculty of Medical Science Universidad de Santiago de Chile, Chile

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German Rehren

15Technology Transfer and Licensing office, Universidad Austral de Chile, Valdivia, Chile

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David Schwefel

16Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany