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Mouse thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro

Yu Lin, Qian Fang, Yue He, Xiaowen Gong, Yinjuan Wang, Ajuan Liang, Guishuan Wang, Shengnan Gong, Ji Wu, Fei Sun
doi: https://doi.org/10.1101/2020.06.15.153668
Yu Lin
1International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Speciality, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China
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Qian Fang
2Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China
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Yue He
1International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Speciality, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China
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Xiaowen Gong
2Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China
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Yinjuan Wang
2Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China
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Ajuan Liang
3Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China
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Guishuan Wang
4Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, China
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Shengnan Gong
4Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, China
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Ji Wu
2Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China
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  • For correspondence: sunfei@shsmu.edu.cn jiwu@sjtu.edu.cn
Fei Sun
1International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Speciality, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China
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  • For correspondence: sunfei@shsmu.edu.cn jiwu@sjtu.edu.cn
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ABSTRACT

The self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Funding information The National Natural Science Foundation of China (81430027, 81720108017, 81671510, 81901536); the National Basic Research Program of China (2014CB943104).

  • ABBREVIATIONS SSCs: spermatogonial stem cells; EVs: extracellular vesicles

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 17, 2020.
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Mouse thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro
Yu Lin, Qian Fang, Yue He, Xiaowen Gong, Yinjuan Wang, Ajuan Liang, Guishuan Wang, Shengnan Gong, Ji Wu, Fei Sun
bioRxiv 2020.06.15.153668; doi: https://doi.org/10.1101/2020.06.15.153668
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Mouse thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro
Yu Lin, Qian Fang, Yue He, Xiaowen Gong, Yinjuan Wang, Ajuan Liang, Guishuan Wang, Shengnan Gong, Ji Wu, Fei Sun
bioRxiv 2020.06.15.153668; doi: https://doi.org/10.1101/2020.06.15.153668

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