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Enhanced recovery of CD9-positive extracellular vesicles from human specimens by chelating reagent

Ayako Kurimoto, Yuki Kawasaki, Toshiki Ueda, Tatsutoshi Inuzuka
doi: https://doi.org/10.1101/2020.06.17.155861
Ayako Kurimoto
Research Department, Miraca Research Institute G.K., Tokyo, Japan
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Yuki Kawasaki
Research Department, Miraca Research Institute G.K., Tokyo, Japan
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Toshiki Ueda
Research Department, Miraca Research Institute G.K., Tokyo, Japan
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Tatsutoshi Inuzuka
Research Department, Miraca Research Institute G.K., Tokyo, Japan
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  • For correspondence: tatsutoshi.inuzuka@miraca.com
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Abstract

Extracellular vesicles (EVs) have gained attention as potential targets of early diagnostics and prognosis in the field of liquid biopsy. Despite clinical potentials, the best method to isolate EVs from specimens remains controversial due to low purity, low specificity, and lack of reproducibility with current isolation methods. Here we show that a chelating reagent enhances the recovery efficiency of EVs from crude biological samples by immunoprecipitation using an anti-CD9 antibody. Proteomic and western blotting analyses show that the EVs isolated using the chelating reagent contain a wider variety of proteins than those isolated with PBS.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 18, 2020.
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Enhanced recovery of CD9-positive extracellular vesicles from human specimens by chelating reagent
Ayako Kurimoto, Yuki Kawasaki, Toshiki Ueda, Tatsutoshi Inuzuka
bioRxiv 2020.06.17.155861; doi: https://doi.org/10.1101/2020.06.17.155861
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Enhanced recovery of CD9-positive extracellular vesicles from human specimens by chelating reagent
Ayako Kurimoto, Yuki Kawasaki, Toshiki Ueda, Tatsutoshi Inuzuka
bioRxiv 2020.06.17.155861; doi: https://doi.org/10.1101/2020.06.17.155861

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