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Electrophysiological validation of premotor interneurons monosynaptically connected to the aCC motoneuron in the Drosophila larval CNS

Carlo N. G. Giachello, Aref Arzan Zarin, Hiroshi Kohsaka, Yuen Ngan Fan, Akinao Nose, Matthias Landgraf, Richard A. Baines
doi: https://doi.org/10.1101/2020.06.17.156430
Carlo N. G. Giachello
1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK
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Aref Arzan Zarin
2Department of Biology, Texas A&M, University, College Station, United States
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Hiroshi Kohsaka
3Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japan
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Yuen Ngan Fan
1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK
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Akinao Nose
3Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japan
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Matthias Landgraf
4Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK
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Richard A. Baines
1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK
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  • For correspondence: Richard.Baines@manchester.ac.uk
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Abstract

Mapping the wired connectivity of a nervous system is a prerequisite for full understanding of function. In this respect, such endeavours can be likened to genome sequencing projects. These projects similarly produce impressive amounts of data which, whilst a technical tour-de-force, remain under-utilised without validation. Validation of neuron synaptic connectivity requires electrophysiology which has the necessary temporal and spatial resolution to map synaptic connectivity. However, this technique is not common and requires extensive equipment and training to master, particularly when applied to the small CNS of the Drosophila larva. Thus, validation of connectivity in this CNS has been more reliant on behavioural analyses and, in particular, activity imaging using the calcium-sensor GCaMP. Whilst both techniques are powerful, they each have significant limitations for this purpose. Here we use electrophysiology to validate an array of driver lines reported to label specific premotor interneurons that the Drosophila connectome project suggests are monosynaptically connected to an identified motoneuron termed the anterior corner cell (aCC). Our results validate this proposition for four selected lines. Thus, in addition to validating the connectome with respect to these four premotor interneurons, our study highlights the need to functionally validate driver lines prior to use.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 19, 2020.
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Electrophysiological validation of premotor interneurons monosynaptically connected to the aCC motoneuron in the Drosophila larval CNS
Carlo N. G. Giachello, Aref Arzan Zarin, Hiroshi Kohsaka, Yuen Ngan Fan, Akinao Nose, Matthias Landgraf, Richard A. Baines
bioRxiv 2020.06.17.156430; doi: https://doi.org/10.1101/2020.06.17.156430
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Electrophysiological validation of premotor interneurons monosynaptically connected to the aCC motoneuron in the Drosophila larval CNS
Carlo N. G. Giachello, Aref Arzan Zarin, Hiroshi Kohsaka, Yuen Ngan Fan, Akinao Nose, Matthias Landgraf, Richard A. Baines
bioRxiv 2020.06.17.156430; doi: https://doi.org/10.1101/2020.06.17.156430

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