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Trophic cooperation promotes bacterial survival of Staphylococcus aureus and Pseudomonas aeruginosa

View ORCID ProfileLaura Camus, View ORCID ProfilePaul Briaud, View ORCID ProfileSylvère Bastien, View ORCID ProfileSylvie Elsen, View ORCID ProfileAnne Doléans-Jordheim, View ORCID ProfileFrançois Vandenesch, View ORCID ProfileKaren Moreau
doi: https://doi.org/10.1101/2020.06.17.156968
Laura Camus
1CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France
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Paul Briaud
1CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France
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Sylvère Bastien
1CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France
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Sylvie Elsen
2Université Grenoble Alpes, CNRS ERL5261, CEA-IRIG-BCI, INSERM UMR1036, Grenoble 38000, France
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Anne Doléans-Jordheim
3Institut des agents infectieux, Hospices Civils de Lyon, Lyon, France
4Bactéries Pathogènes Opportunistes et Environnement, UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1 & VetAgro Sup, Villeurbanne, France
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François Vandenesch
1CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France
3Institut des agents infectieux, Hospices Civils de Lyon, Lyon, France
5Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France
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Karen Moreau
1CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France
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  • For correspondence: karen.moreau@univ-lyon1.fr
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Abstract

In the context of infection, Pseudomonas aeruginosa and Staphylococcus aureus are frequently co-isolated, particularly in cystic fibrosis (CF) patients. Within lungs, the two pathogens exhibit a range of competitive and coexisting interactions. In the present study, we explored the impact of S. aureus on the physiology of P. aeruginosa in the context of coexistence. Transcriptomic analyses showed that S. aureus significantly and specifically affects the expression of numerous genes involved in P. aeruginosa carbon and amino acid metabolism. In particular, 65% of the strains presented considerable overexpression of the genes involved in the acetoin catabolic (aco) pathway. We demonstrated that acetoin is (i) produced by clinical S. aureus strains, (ii) detected in sputa from CF patients, and (iii) involved in P. aeruginosa’s aco system induction. Furthermore, acetoin is catabolized by P. aeruginosa, a metabolic process that improves the survival of both pathogens by providing a new carbon source for P. aeruginosa and avoiding the toxic accumulation of acetoin on S. aureus. Due to its beneficial effects on both bacteria, acetoin catabolism could testify to the establishment of trophic cooperation between S. aureus and P. aeruginosa in the CF lung environment, thus promoting their persistence.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • All authors declare no competing interests.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 18, 2020.
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Trophic cooperation promotes bacterial survival of Staphylococcus aureus and Pseudomonas aeruginosa
Laura Camus, Paul Briaud, Sylvère Bastien, Sylvie Elsen, Anne Doléans-Jordheim, François Vandenesch, Karen Moreau
bioRxiv 2020.06.17.156968; doi: https://doi.org/10.1101/2020.06.17.156968
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Trophic cooperation promotes bacterial survival of Staphylococcus aureus and Pseudomonas aeruginosa
Laura Camus, Paul Briaud, Sylvère Bastien, Sylvie Elsen, Anne Doléans-Jordheim, François Vandenesch, Karen Moreau
bioRxiv 2020.06.17.156968; doi: https://doi.org/10.1101/2020.06.17.156968

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