ABSTRACT
Type IV secreted effectors (T4SEs) can be translocated into the cytosol of host cells via type IV secretion system (T4SS) and cause diseases. However, experimental approaches to identify T4SEs are time- and resource-consuming, and the existing computational tools based on machine learning techniques have some obvious limitations such as the lack of interpretability in the prediction models. In this study, we proposed a new model, T4SE-XGB, which uses the eXtreme gradient boosting (XGBoost) algorithm for accurate identification of type IV effectors based on optimal protein sequence features. After trying 20 different features, the best result achieved when all features were fed into XGBoost by the 5-fold cross validation compared with different machine learning methods. Then, the ReliefF algorithm was adopted to optimize feature vectors and got final 1100 features for our dataset which obviously improved the model performance. T4SE-XGB exhibited highest predictive performance on the independent test set and clearly outperforms other recent prediction tools. What’s more, the SHAP method was used to interpret the contribution of features to model predictions. The identification of key features can contribute to an improved understanding of multifactorial contributors to host-pathogen interactions and bacterial pathogenesis. In addition to type IV effector prediction, we believe that the proposed framework composed of model construction and model interpretation can provide more instructive guidance for further research of developing novel computational methods and mechanism exploration of biological problems. The data and code for this study can be found at https://github.com/CT001002/T4SE-XGB.
Competing Interest Statement
The authors have declared no competing interest.