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FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner

View ORCID ProfileSumana Sundaramurthy, SarahBeth Votra, Arianna Laszlo, View ORCID ProfileTim Davies, View ORCID ProfileDavid Pruyne
doi: https://doi.org/10.1101/2020.06.18.159582
Sumana Sundaramurthy
1Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY
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SarahBeth Votra
1Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY
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Arianna Laszlo
1Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY
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Tim Davies
2Department of Pathology and Cell Biology, Columbia University, New York, NY
3Department of Biosciences, Durham University, Durham, UK
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David Pruyne
1Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY
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  • For correspondence: pruyned@upstate.edu
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Abstract

The striated body wall muscles of Caenorhabditis elegans are a simple model system with well-characterized sarcomeres that have many vertebrate protein homologs. Previously, we observed deletion mutants for two formin genes, fhod-1 and cyk-1, developed thin muscles with abnormal dense bodies/sarcomere Z-lines. However, the nature of the cyk-1 mutation necessitated maternal CYK-1 expression for viability of the examined animals. Here, we tested the effects of complete loss of CYK-1 using a fast acting temperature-sensitive cyk-1(ts) mutant. Surprisingly, neither post-embryonic loss of CYK-1 nor acute loss of CYK-1 during embryonic sarcomerogenesis caused muscle defects, suggesting CYK-1 might not play a direct role in muscle development. Consistent with this, examination of cyk-1(Δ) mutants re-expressing CYK-1 in a mosaic pattern showed CYK-1 cannot rescue muscle defects in a muscle cell autonomous manner, suggesting muscle phenotypes caused by cyk-1 deletion are likely indirect. Conversely, mosaic re-expression of FHOD-1 in fhod-1(Δ) mutants promoted muscle cell growth, as well as proper Z-line organization, in a muscle cell autonomous manner. As we can observe no effect of loss of any other worm formin on muscle development, we conclude that FHOD-1 is the only formin that directly promotes striated muscle development in C. elegans.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 19, 2020.
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FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner
Sumana Sundaramurthy, SarahBeth Votra, Arianna Laszlo, Tim Davies, David Pruyne
bioRxiv 2020.06.18.159582; doi: https://doi.org/10.1101/2020.06.18.159582
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FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner
Sumana Sundaramurthy, SarahBeth Votra, Arianna Laszlo, Tim Davies, David Pruyne
bioRxiv 2020.06.18.159582; doi: https://doi.org/10.1101/2020.06.18.159582

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