Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network

View ORCID ProfileJoshua Hutchings, Viktoriya G. Stancheva, Nick R. Brown, View ORCID ProfileAlan M.C. Cheung, View ORCID ProfileElizabeth A. Miller, View ORCID ProfileGiulia Zanetti
doi: https://doi.org/10.1101/2020.06.18.159608
Joshua Hutchings
1Institute of Structural and Molecular Biology, Birkbeck College, London
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joshua Hutchings
Viktoriya G. Stancheva
2MRC Laboratory of Molecular Biology, Cambridge
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nick R. Brown
1Institute of Structural and Molecular Biology, Birkbeck College, London
3The Francis Crick Institute, London
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alan M.C. Cheung
1Institute of Structural and Molecular Biology, Birkbeck College, London
4School of Biochemistry at the University of Bristol
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alan M.C. Cheung
Elizabeth A. Miller
2MRC Laboratory of Molecular Biology, Cambridge
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Elizabeth A. Miller
Giulia Zanetti
1Institute of Structural and Molecular Biology, Birkbeck College, London
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Giulia Zanetti
  • For correspondence: g.zanetti@bbk.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Summary

The COPII coat mediates Endoplasmic Reticulum (ER) to Golgi trafficking of thousands of proteins. Five essential coat components assemble on the ER membrane to induce the formation of transport vesicles while selectively recruiting cargo proteins. Individual COPII proteins interact dynamically through multiple interfaces to assemble into inner and outer coat layers, forming coats of varied architectures. Here we have used cryo-electron tomography (cryo-ET) and subtomogram averaging to visualise the complete membrane-bound COPII coat to unprecedented detail, revealing a complex interaction network that includes a number of previously unknown interfaces. We identify essential interactions, and functionally characterise their contribution to COPII assembly and membrane remodelling. We find that multiple finely-tuned interfaces contribute to coat organization required to remodel membranes, depending additionally on membrane properties. Our work sheds light on how COPII transports the diverse range of cargo molecules required by the cell.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted June 24, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network
Joshua Hutchings, Viktoriya G. Stancheva, Nick R. Brown, Alan M.C. Cheung, Elizabeth A. Miller, Giulia Zanetti
bioRxiv 2020.06.18.159608; doi: https://doi.org/10.1101/2020.06.18.159608
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network
Joshua Hutchings, Viktoriya G. Stancheva, Nick R. Brown, Alan M.C. Cheung, Elizabeth A. Miller, Giulia Zanetti
bioRxiv 2020.06.18.159608; doi: https://doi.org/10.1101/2020.06.18.159608

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4105)
  • Biochemistry (8808)
  • Bioengineering (6509)
  • Bioinformatics (23446)
  • Biophysics (11784)
  • Cancer Biology (9199)
  • Cell Biology (13314)
  • Clinical Trials (138)
  • Developmental Biology (7430)
  • Ecology (11403)
  • Epidemiology (2066)
  • Evolutionary Biology (15143)
  • Genetics (10430)
  • Genomics (14036)
  • Immunology (9167)
  • Microbiology (22142)
  • Molecular Biology (8802)
  • Neuroscience (47539)
  • Paleontology (350)
  • Pathology (1427)
  • Pharmacology and Toxicology (2489)
  • Physiology (3729)
  • Plant Biology (8076)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2220)
  • Systems Biology (6036)
  • Zoology (1252)