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Evaluation of cytotoxic, antiviral effect and mutagenic potential of a micronutrient combination in vitro cell culture

Nazish Matti, Muhammad Ashraf, Muhammad Adil Rasheed, Imran Altaf, Isabel Carvalho, View ORCID ProfileMuhammad Faisal Nadeem
doi: https://doi.org/10.1101/2020.06.18.160333
Nazish Matti
1Department of Pharmacology and Toxicology, University of Veterinary & Animal Sciences, Lahore
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Muhammad Ashraf
2Department of Pharmacology and Toxicology, University of Veterinary & Animal Sciences, Lahore
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Muhammad Adil Rasheed
3Assistant Professor, Department of Pharmacology and Toxicology, University of Veterinary & Animal Sciences, Lahore
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Imran Altaf
4Institute of Pharmaceutical Sciences, Lahore
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Isabel Carvalho
5Assistant Professor Department of Microbiology, University of Veterinary & Animal Sciences, Lahore
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Muhammad Faisal Nadeem
6Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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  • ORCID record for Muhammad Faisal Nadeem
  • For correspondence: Faisal.nadeem@uvas.edu.pk
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Abstract

Background Micronutrients are essential for the body to produce enzymes, hormones and other substances essential for proper growth and development. Iodine, potassium iodide and ascorbic acid are ones of the most important in global public health terms. However, their lack represents a major threat to the human and animal health.

Methodology Antiviral, cytotoxic and mutagenic activity of commercially available micronutrient combination consisting of iodine, ascorbic acid, potassium iodide and excipients was evaluated. Commercial preparation of micronutrient combination was compared with self-prepared micronutrient preparation. MTT assay was used to evaluate the cytotoxicity of all the preparation. Confluent monolayer of Chicken Embryo Fibroblasts grown in 96-well cell culture plates were treated with ten concentrations of each preparation in triplicate manner and was used to determine the viability of the cells and cell survival percentage. Antiviral efficacy was determined against influenza virus H9N1 strain by virus infection and subsequent cell viability assay. Furthermore, mutagenicity was measured by bacterial reverse mutation analysis by Ames test using two strains of Salmonella typhimurium TA100 and TA 98 with and without S9. After appropriate incubation, number of revertant colonies per plate were counted in triplicate manner and MF was calculated.

Results Our combination showed cytotoxicity at doses higher than 11.71ug/ml while showed significant antiviral efficacy at concentrations of 11.71ug/ml, 5.86ug/ml, 2.93ug/ml and 1.4ug/ml which faded away at lower dilutions. Commercially available preparation was found to be non-mutagenic in our experiment.

Conclusions Combination consisting of iodine, ascorbic acid, potassium iodide is effective in the treatment of viral infections.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted June 20, 2020.
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Evaluation of cytotoxic, antiviral effect and mutagenic potential of a micronutrient combination in vitro cell culture
Nazish Matti, Muhammad Ashraf, Muhammad Adil Rasheed, Imran Altaf, Isabel Carvalho, Muhammad Faisal Nadeem
bioRxiv 2020.06.18.160333; doi: https://doi.org/10.1101/2020.06.18.160333
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Evaluation of cytotoxic, antiviral effect and mutagenic potential of a micronutrient combination in vitro cell culture
Nazish Matti, Muhammad Ashraf, Muhammad Adil Rasheed, Imran Altaf, Isabel Carvalho, Muhammad Faisal Nadeem
bioRxiv 2020.06.18.160333; doi: https://doi.org/10.1101/2020.06.18.160333

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