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Nanoparticle Tracking Analysis: A powerful tool for characterizing magnetosome preparations

View ORCID ProfileAlfred Fernández-Castané, Hong Li, Stephan Joseph, Moritz Ebeler, Matthias Franzreb, Daniel G. Bracewell, Tim W. Overton, Owen R.T. Thomas
doi: https://doi.org/10.1101/2020.06.23.166587
Alfred Fernández-Castané
1School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK
2Institute for Microbiology & Infection, University of Birmingham, Birmingham, B15 2TT, UK
3Aston Institute of Materials Research, Aston University, Birmingham, B4 7ET, UK
4Energy and Bioproducts Research Institute, Aston University, Birmingham, B4 7ET, UK
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  • ORCID record for Alfred Fernández-Castané
  • For correspondence: a.fernandez-castane1@aston.ac.uk o.r.t.thomas@bham.ac.uk
Hong Li
1School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK
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Stephan Joseph
1School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK
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Moritz Ebeler
5Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany
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Matthias Franzreb
5Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany
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Daniel G. Bracewell
6Department of Biochemical Engineering, University College London, London, WC1E 6BT, UK
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Tim W. Overton
1School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK
2Institute for Microbiology & Infection, University of Birmingham, Birmingham, B15 2TT, UK
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Owen R.T. Thomas
1School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK
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  • For correspondence: a.fernandez-castane1@aston.ac.uk o.r.t.thomas@bham.ac.uk
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Abstract

Nanoparticle Tracking Analysis (NTA) has been employed to measure the particle concentration and size distribution of magnetosomes extracted and purified from Magnetospirillum gryphiswaldense MSR-1, and then exposed to probe ultrasonication for various times, or 1% (w/v) sodium dodecyl sulphate (SDS) for 1 h. Particle concentration increased 3.7-fold over the first 15 min of ultrasonication (from 2 × 108 to >7.3 × 108 particles mL−1), but fell steeply to ~3.6 × 108 particles mL−1 after 20 min. NTA of untreated magnetosome preparation confirmed a wide particle distribution dominated by larger species (D[1,0] = 312 nm; Dn50 = 261 nm; mode = 243 nm) with no particles in the size range of isolated single magnetosomes. After 5 min of ultrasonication the whole particle size distribution shifted to smaller size (D[1,0] = 133 nm; Dn50 = 99 nm; mode = 36 nm, corresponding to individual magnetosomes), but longer treatment times (15 and 20 min) reversed the previous transition; all characteristic numbers of the particle size distributions increased and very few small particles were detected. Side-by-side comparison of NTA and TEM sizing data revealed remarkable similarity at low ultrasonication times, with both showing single magnetosomes accounted for ~30% population after 5 min. Exposure of magnetosomes to SDS resulted in a ~3-fold increase in particle concentration to 5.8 × 108 particles mL−1, narrowing of the size distribution and gross elimination of particles below 60 nm. We conclude that NTA is a rapid cost-effective technique for measuring particle number, size distribution and aggregation state of magnetosomes in solution, but requires further work to improve its resolving power.

Competing Interest Statement

The authors have declared no competing interest.

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Posted June 24, 2020.
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Nanoparticle Tracking Analysis: A powerful tool for characterizing magnetosome preparations
Alfred Fernández-Castané, Hong Li, Stephan Joseph, Moritz Ebeler, Matthias Franzreb, Daniel G. Bracewell, Tim W. Overton, Owen R.T. Thomas
bioRxiv 2020.06.23.166587; doi: https://doi.org/10.1101/2020.06.23.166587
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Nanoparticle Tracking Analysis: A powerful tool for characterizing magnetosome preparations
Alfred Fernández-Castané, Hong Li, Stephan Joseph, Moritz Ebeler, Matthias Franzreb, Daniel G. Bracewell, Tim W. Overton, Owen R.T. Thomas
bioRxiv 2020.06.23.166587; doi: https://doi.org/10.1101/2020.06.23.166587

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