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The buds of Oscarella lobularis (Porifera): a new convenient model for sponge cell and developmental biology

Rocher Caroline, Vernale Amélie, Fierro-Constaín Laura, Séjourné Nina, Chenesseau Sandrine, Marschal Christian, Le Goff Emilie, Dutilleul Morgan, Matthews Cédric, Marschal Florent, Brouilly Nicolas, Massey-Harroche Dominique, Ereskovsky Alexander, Le Bivic André, Borchiellini Carole, View ORCID ProfileRenard Emmanuelle
doi: https://doi.org/10.1101/2020.06.23.167296
Rocher Caroline
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Vernale Amélie
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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Fierro-Constaín Laura
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Séjourné Nina
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Chenesseau Sandrine
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Marschal Christian
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Le Goff Emilie
3ISEM, Université de Montpellier, CNRS, IRD, EPHE, Montpellier, France
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Dutilleul Morgan
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Matthews Cédric
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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Marschal Florent
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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Brouilly Nicolas
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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Massey-Harroche Dominique
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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Ereskovsky Alexander
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
4Saint-Petersburg State University, Saint-Petersburg, Russia
5Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia
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Le Bivic André
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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Borchiellini Carole
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
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  • For correspondence: Carole.borchiellini@imbe.fr Emmanuelle.renard@univ-amu.fr
Renard Emmanuelle
1Aix Marseille Univ, CNRS, IRD, IMBE UMR 7263, Avignon Université, Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, Station Marine d’Endoume, Marseille, France
2Aix Marseille Univ, CNRS, Institute of Developmental Biology of Marseille (IBDM), case 907, 13288, Marseille cedex 09, France
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  • ORCID record for Renard Emmanuelle
  • For correspondence: Carole.borchiellini@imbe.fr Emmanuelle.renard@univ-amu.fr
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Abstract

The comparative study of the four non-bilaterian phyla (Cnidaria, Placozoa, Ctenophora, Porifera) should provide insights into the origin of bilaterian traits. Except for Cnidaria, present knowledge on the cell biology and development of these animals is so far limited. Non-bilaterian models are needed to get further into cell architecture and molecular mechanisms.

Given the developmental, histological, ecological and genomic differences between the four sponge classes, we develop a new sponge model: the buds of the Oscarella lobularis (class Homoscleromorpha). This experimental model supplements the two other most famous sponge models Amphimedon queenslandica and Ephydatia muelleri, both belonging to the class Demospongiae.

Budding is a natural and spontaneous asexual reproduction mean, but budding can be triggered in vitro ensuring availability of biological material all year long. We provide a full description of buds, from their formation to their development into juveniles. Their transparency enables fluorescent and live imaging, and their abundance allows for experimental replicates. Moreover, regeneration and cell reaggregation capabilities provide interesting experimental morphogenetic contexts. The numerous techniques now mastered on these buds make it a new suitable sponge model.

Summary statement Studying sponge biology is needed to understand the evolution of metazoans. We developed a new model suitable for experimental biology that allows to study morphogenetic processes with modern tools.

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  • ↵* co-last authors

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Posted June 23, 2020.
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The buds of Oscarella lobularis (Porifera): a new convenient model for sponge cell and developmental biology
Rocher Caroline, Vernale Amélie, Fierro-Constaín Laura, Séjourné Nina, Chenesseau Sandrine, Marschal Christian, Le Goff Emilie, Dutilleul Morgan, Matthews Cédric, Marschal Florent, Brouilly Nicolas, Massey-Harroche Dominique, Ereskovsky Alexander, Le Bivic André, Borchiellini Carole, Renard Emmanuelle
bioRxiv 2020.06.23.167296; doi: https://doi.org/10.1101/2020.06.23.167296
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The buds of Oscarella lobularis (Porifera): a new convenient model for sponge cell and developmental biology
Rocher Caroline, Vernale Amélie, Fierro-Constaín Laura, Séjourné Nina, Chenesseau Sandrine, Marschal Christian, Le Goff Emilie, Dutilleul Morgan, Matthews Cédric, Marschal Florent, Brouilly Nicolas, Massey-Harroche Dominique, Ereskovsky Alexander, Le Bivic André, Borchiellini Carole, Renard Emmanuelle
bioRxiv 2020.06.23.167296; doi: https://doi.org/10.1101/2020.06.23.167296

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