Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

POMC-specific knockdown of Tril reduces body adiposity and increases hypothalamic leptin responsiveness

Alexandre Moura-Assis, Pedro A. Nogueira, View ORCID ProfileJose C. de-Lima-Junior, Fernando M. Simabuco, Joana M. Gaspar, Jose Donato Jr, Licio A. Velloso
doi: https://doi.org/10.1101/2020.06.25.172379
Alexandre Moura-Assis
1Laboratory of Cell Signalling-Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pedro A. Nogueira
1Laboratory of Cell Signalling-Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jose C. de-Lima-Junior
1Laboratory of Cell Signalling-Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jose C. de-Lima-Junior
Fernando M. Simabuco
2Multidisciplinary Laboratory of Food and Health (LABMAS), School of Applied Sciences (FCA), University of Campinas (UNICAMP), Limeira, São Paulo, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joana M. Gaspar
1Laboratory of Cell Signalling-Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jose Donato Jr
3Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Licio A. Velloso
1Laboratory of Cell Signalling-Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lavelloso@unicamp.br
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

In a public dataset of transcripts differentially expressed in selected neuronal subpopulations of the arcuate nucleus, we identified TLR4-interactor with leucine-rich repeats (Tril) as a potential candidate for mediating the harmful effects of a high-fat diet in proopiomelanocortin (POMC) neurons. The non-cell-specific inhibition of Tril in the arcuate nucleus resulted in reduced hypothalamic inflammation, protection against diet-induced obesity associated with increased whole-body energy expenditure and increased systemic glucose tolerance. The inhibition of Tril, specifically in POMC neurons, resulted in a trend for protection against diet-induced obesity, increased energy expenditure and increased hypothalamic sensitivity to leptin. Thus, Tril emerges as a new component of the complex mechanisms that promote hypothalamic dysfunction in experimental diet-induced obesity.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Conflict of interests: The authors declare that no conflicts of interests exists.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 27, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
POMC-specific knockdown of Tril reduces body adiposity and increases hypothalamic leptin responsiveness
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
POMC-specific knockdown of Tril reduces body adiposity and increases hypothalamic leptin responsiveness
Alexandre Moura-Assis, Pedro A. Nogueira, Jose C. de-Lima-Junior, Fernando M. Simabuco, Joana M. Gaspar, Jose Donato Jr, Licio A. Velloso
bioRxiv 2020.06.25.172379; doi: https://doi.org/10.1101/2020.06.25.172379
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
POMC-specific knockdown of Tril reduces body adiposity and increases hypothalamic leptin responsiveness
Alexandre Moura-Assis, Pedro A. Nogueira, Jose C. de-Lima-Junior, Fernando M. Simabuco, Joana M. Gaspar, Jose Donato Jr, Licio A. Velloso
bioRxiv 2020.06.25.172379; doi: https://doi.org/10.1101/2020.06.25.172379

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (2533)
  • Biochemistry (4977)
  • Bioengineering (3486)
  • Bioinformatics (15232)
  • Biophysics (6910)
  • Cancer Biology (5395)
  • Cell Biology (7753)
  • Clinical Trials (138)
  • Developmental Biology (4539)
  • Ecology (7159)
  • Epidemiology (2059)
  • Evolutionary Biology (10234)
  • Genetics (7517)
  • Genomics (9794)
  • Immunology (4863)
  • Microbiology (13234)
  • Molecular Biology (5144)
  • Neuroscience (29465)
  • Paleontology (203)
  • Pathology (838)
  • Pharmacology and Toxicology (1466)
  • Physiology (2142)
  • Plant Biology (4756)
  • Scientific Communication and Education (1013)
  • Synthetic Biology (1338)
  • Systems Biology (4014)
  • Zoology (768)