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Mesoscopic cortical network reorganization during recovery of partial optic nerve injury in GCaMP6s mice

Marianne Groleau, Mojtaba Nazari-Ahangarkolaee, View ORCID ProfileMatthieu P. Vanni, Jacqueline L. Higgins, Anne-Sophie Vézina Bédard, View ORCID ProfileBernhard A. Sabel, View ORCID ProfileMajid H. Mohajerani, View ORCID ProfileElvire Vaucher
doi: https://doi.org/10.1101/2020.06.28.173799
Marianne Groleau
1Laboratoire de Neurobiologie de la Cognition Visuelle, École d’Optométrie, Université de Montréal, Montréal, QC, Canada, H3T 1P1
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Mojtaba Nazari-Ahangarkolaee
2Department of Neuroscience, Canadian Centre for Behavioural Neuroscience (CCBN), University of Lethbridge, Lethbridge, AB, Canada, T1K 3M4
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Matthieu P. Vanni
3Laboratoire de Neurophotonique, École d’Optométrie, Université de Montréal, Montréal, QC, Canada, H3T 1P1
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  • ORCID record for Matthieu P. Vanni
Jacqueline L. Higgins
1Laboratoire de Neurobiologie de la Cognition Visuelle, École d’Optométrie, Université de Montréal, Montréal, QC, Canada, H3T 1P1
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Anne-Sophie Vézina Bédard
1Laboratoire de Neurobiologie de la Cognition Visuelle, École d’Optométrie, Université de Montréal, Montréal, QC, Canada, H3T 1P1
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Bernhard A. Sabel
5Institute of Medical Psychology, Medical Faculty, Otto-v.-Guericke University of Magdeburg, Magdeburg, 39120, Germany
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Majid H. Mohajerani
2Department of Neuroscience, Canadian Centre for Behavioural Neuroscience (CCBN), University of Lethbridge, Lethbridge, AB, Canada, T1K 3M4
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  • For correspondence: elvire.vaucher@umontreal.ca mohajerani@uleth.ca
Elvire Vaucher
1Laboratoire de Neurobiologie de la Cognition Visuelle, École d’Optométrie, Université de Montréal, Montréal, QC, Canada, H3T 1P1
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  • ORCID record for Elvire Vaucher
  • For correspondence: elvire.vaucher@umontreal.ca mohajerani@uleth.ca
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Abstract

As the residual vision following a traumatic optic nerve injury can spontaneously recover over time, we explored the plasticity of cortical networks during the early post-optic nerve crush (ONC) phase. Using in vivo wide-field calcium imaging on awake Thy1-GCaMP6s mice, we characterized resting state and evoked cortical activity before, during, and 30 days after ONC. The recovery of monocular visual acuity and depth perception was evaluated at the same time points. Cortical responses to an LED flash decreased in the contralateral hemisphere in the primary visual cortex and in the secondary visual areas following the ONC, but was partially rescued between 3 and 5 days post-ONC, remaining stable thereafter. The connectivity between visual and non-visual regions was disorganized after the crush, as shown by a decorrelation, but correlated activity was restored 30 days after the injury. The number of surviving retinal ganglion cells dramatically dropped and remained low. At the behavioral level, the ONC resulted in visual acuity loss on the injured side and an increase in visual acuity with the non-injured eye. In conclusion, our results show a reorganization of connectivity between visual and associative cortical areas after an ONC, which is indicative of spontaneous cortical plasticity.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted June 28, 2020.
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Mesoscopic cortical network reorganization during recovery of partial optic nerve injury in GCaMP6s mice
Marianne Groleau, Mojtaba Nazari-Ahangarkolaee, Matthieu P. Vanni, Jacqueline L. Higgins, Anne-Sophie Vézina Bédard, Bernhard A. Sabel, Majid H. Mohajerani, Elvire Vaucher
bioRxiv 2020.06.28.173799; doi: https://doi.org/10.1101/2020.06.28.173799
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Mesoscopic cortical network reorganization during recovery of partial optic nerve injury in GCaMP6s mice
Marianne Groleau, Mojtaba Nazari-Ahangarkolaee, Matthieu P. Vanni, Jacqueline L. Higgins, Anne-Sophie Vézina Bédard, Bernhard A. Sabel, Majid H. Mohajerani, Elvire Vaucher
bioRxiv 2020.06.28.173799; doi: https://doi.org/10.1101/2020.06.28.173799

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