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Cellular logics bringing the symmetry breaking in spiral nucleation revealed by trans-scale imaging

Taishi Kakizuka, View ORCID ProfileYusuke Hara, Yusaku Ohta, Asuka Mukai, Aya Ichiraku, Yoshiyuki Arai, Taro Ichimura, Takeharu Nagai, Kazuki Horikawa
doi: https://doi.org/10.1101/2020.06.29.176891
Taishi Kakizuka
1Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
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Yusuke Hara
2Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan
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  • ORCID record for Yusuke Hara
Yusaku Ohta
2Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan
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Asuka Mukai
2Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan
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Aya Ichiraku
2Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan
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Yoshiyuki Arai
3Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan
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Taro Ichimura
1Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
4PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
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Takeharu Nagai
1Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
3Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan
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Kazuki Horikawa
2Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima City, Tokushima 770-8503, Japan
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  • For correspondence: horikawa.kazuki@tokushima-u.ac.jp
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Summary

The spiral wave is a commonly observed spatio-temporal order in diverse signal relaying systems. Although properties of generated spirals have been well studied, the mechanisms for their spontaneous generation in living systems remain elusive. By the newly developed imaging system for trans-scale observation of the intercellular communication among ∼130,000 cells of social amoeba, we investigated the onset dynamics of cAMP signaling and identified mechanisms for the self-organization of the spiral wave at three distinct scalings: At the population-level, the structured heterogeneity of excitability fragments traveling waves at its high/low boundary, that becomes the generic source of the spiral wave. At the cell-level, both the pacemaking leaders and pulse-amplifying followers regulate the heterogeneous growth of the excitability. At the intermediate-scale, the essence of the spontaneous wave fragmentation is the asymmetric positioning of the pacemakers in the high-excitability territories, whose critical controls are operated by a small number of cells, pulse counts, and pulse amounts.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 29, 2020.
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Cellular logics bringing the symmetry breaking in spiral nucleation revealed by trans-scale imaging
Taishi Kakizuka, Yusuke Hara, Yusaku Ohta, Asuka Mukai, Aya Ichiraku, Yoshiyuki Arai, Taro Ichimura, Takeharu Nagai, Kazuki Horikawa
bioRxiv 2020.06.29.176891; doi: https://doi.org/10.1101/2020.06.29.176891
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Cellular logics bringing the symmetry breaking in spiral nucleation revealed by trans-scale imaging
Taishi Kakizuka, Yusuke Hara, Yusaku Ohta, Asuka Mukai, Aya Ichiraku, Yoshiyuki Arai, Taro Ichimura, Takeharu Nagai, Kazuki Horikawa
bioRxiv 2020.06.29.176891; doi: https://doi.org/10.1101/2020.06.29.176891

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