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Trans-scale-scope to find rare cellular activity in sub-million cells

View ORCID ProfileTaro Ichimura, View ORCID ProfileTaishi Kakizuka, View ORCID ProfileKazuki Horikawa, Kaoru Seiriki, Atsushi Kasai, View ORCID ProfileHitoshi Hashimoto, View ORCID ProfileKatsumasa Fujita, View ORCID ProfileTomonobu M. Watanabe, View ORCID ProfileTakeharu Nagai
doi: https://doi.org/10.1101/2020.06.29.179044
Taro Ichimura
1Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
2PRESTO, Japan Science and Technology Agency, Tokyo, 113-0033, Japan
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  • For correspondence: ichimura@otri.osaka-u.ac.jp ng1@sanken.osaka-u.ac.jp
Taishi Kakizuka
1Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
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Kazuki Horikawa
3Department of Optical Imaging, Center for Advanced Research Promotion, Tokushima University Graduate School, Kuramoto-cho 3-18-15, Tokushima, Tokushima 770-8503, Japan
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Kaoru Seiriki
4Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka 565-0871, Japan
5Institute for Transdisciplinary Graduate Degree Programs, Osaka University, Yamadaoka 1-1, Suita, Osaka 565-0871, Japan
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Atsushi Kasai
4Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka 565-0871, Japan
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Hitoshi Hashimoto
1Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
4Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka 1-6, Suita, Osaka 565-0871, Japan
6Molecular Research Center for Children’s Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan
7Division of Bioscience, Institute for Datability Science, Osaka University, Yamadaoka 1-1, Suita, Osaka 565-0871, Japan
8Department of Molecular Pharmaceutical Science, Graduate School of Medicine, Osaka University, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan
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Katsumasa Fujita
1Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
9Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
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Tomonobu M. Watanabe
10Laboratory for Comprehensive Bioimaging, RIKEN Center for Biosystems Dynamics Research (BDR), Minatomachi-minami 2-2-3, Chuo-ku, Kobe, Hyogo 650-0047, Japan
11Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan
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Takeharu Nagai
1Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0871, Japan
7Division of Bioscience, Institute for Datability Science, Osaka University, Yamadaoka 1-1, Suita, Osaka 565-0871, Japan
12The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan
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  • For correspondence: ichimura@otri.osaka-u.ac.jp ng1@sanken.osaka-u.ac.jp
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Abstract

There are many phenomena in biological systems where less than 1% of rare cells trigger entire multicellular systems. In order to scientifically understand the mechanisms of these rare cell-driven systems, simultaneous observation of huge populations of cells is essential. It has been difficult to observe the dynamics of such phenomena with conventional microscopes. In this study, we propose a novel type of imager, trans-scale-scope, which allows us to observe cell dynamics with sub-cell resolution in a field of view greater than a centimeter. The imaging system is mainly composed of a hundred-megapixel image sensor and a macro-lens for full-size sensor. This method, which is the basement of the future imaging system named AMATERAS (A Multi-scale/modal Analytical Tool for Every Rare Activity in Singularity), realized observation of sub-million cells at the same time. By observing the macroscale-pattern formation process of Dictyostelium discoideum, a model organism for studying fundamental aspects of cell-cell communication and chemotaxis, we have shown that the 0.1 % of rare cells triggered a stream of collective cell migration.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 30, 2020.
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Trans-scale-scope to find rare cellular activity in sub-million cells
Taro Ichimura, Taishi Kakizuka, Kazuki Horikawa, Kaoru Seiriki, Atsushi Kasai, Hitoshi Hashimoto, Katsumasa Fujita, Tomonobu M. Watanabe, Takeharu Nagai
bioRxiv 2020.06.29.179044; doi: https://doi.org/10.1101/2020.06.29.179044
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Trans-scale-scope to find rare cellular activity in sub-million cells
Taro Ichimura, Taishi Kakizuka, Kazuki Horikawa, Kaoru Seiriki, Atsushi Kasai, Hitoshi Hashimoto, Katsumasa Fujita, Tomonobu M. Watanabe, Takeharu Nagai
bioRxiv 2020.06.29.179044; doi: https://doi.org/10.1101/2020.06.29.179044

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