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Asymmetric neurogenic commitment of retinal progenitors is regulated via the Notch endocytic pathway

View ORCID ProfileElisa Nerli, View ORCID ProfileMauricio Rocha-Martins, View ORCID ProfileCaren Norden
doi: https://doi.org/10.1101/2020.06.30.179499
Elisa Nerli
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
2Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
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Mauricio Rocha-Martins
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
2Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
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  • For correspondence: cnorden@igc.gulbenkian.pt rocha@mpi-cbg.de
Caren Norden
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany
2Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal
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  • For correspondence: cnorden@igc.gulbenkian.pt rocha@mpi-cbg.de
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Abstract

During brain development, progenitor cells need to balance proliferation and differentiation in order to generate different neurons in the correct numbers and proportions. Currently, the patterns of multipotent progenitors’ division that lead to neurogenic entry and the factors that regulate them are not fully understood. We here use the zebrafish retina to address this gap, exploiting the possibilities for quantitative live-imaging. We show that early neurogenic progenitors arise from asymmetric divisions. Notch regulates this asymmetry as when inhibited, symmetric divisions producing two neurogenic progenitors occur. Surprisingly, Notch does not act through an apicobasal activity gradient as previously suggested but through asymmetric inheritance of Sara-positive endosomes. Further, the resulting neurogenic progenitors show cell biological features different from multipotent progenitors, raising the possibility that an intermediate state of progenitors exists in the retina. Our study thus opens new insights into the regulation of proliferative and differentiative events during central nervous system development.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 01, 2020.
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Asymmetric neurogenic commitment of retinal progenitors is regulated via the Notch endocytic pathway
Elisa Nerli, Mauricio Rocha-Martins, Caren Norden
bioRxiv 2020.06.30.179499; doi: https://doi.org/10.1101/2020.06.30.179499
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Asymmetric neurogenic commitment of retinal progenitors is regulated via the Notch endocytic pathway
Elisa Nerli, Mauricio Rocha-Martins, Caren Norden
bioRxiv 2020.06.30.179499; doi: https://doi.org/10.1101/2020.06.30.179499

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