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DIRT/3D: 3D phenotyping for maize (Zea mays) root architecture in the field

Suxing Liu, Carlos Sherard Barrow, View ORCID ProfileMeredith Hanlon, View ORCID ProfileJonathan P. Lynch, View ORCID ProfileAlexander Bucksch
doi: https://doi.org/10.1101/2020.06.30.180059
Suxing Liu
1Department of Plant Biology, University of Georgia, Athens, GA, United States
2Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, United States
3Institute of Bioinformatics, University of Georgia, Athens, GA, United States
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Carlos Sherard Barrow
4Instrumentation Shop, University of Georgia, Athens, GA, United States
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Meredith Hanlon
5Department of Plant Science, Penn State University, State College, PA, United States
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Jonathan P. Lynch
5Department of Plant Science, Penn State University, State College, PA, United States
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Alexander Bucksch
1Department of Plant Biology, University of Georgia, Athens, GA, United States
2Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, United States
3Institute of Bioinformatics, University of Georgia, Athens, GA, United States
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  • For correspondence: bucksch@uga.edu
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Abstract

The development of crops with deeper roots holds substantial promise to mitigate the consequences of climate change. Deeper roots are an essential factor to improve water uptake as a way to enhance crop resilience to drought, to increase nitrogen capture to reduce fertilizer inputs and to increase carbon sequestration from the atmosphere to improve soil organic fertility. A major obstacle to achieving these improvements is the bottleneck in the high-throughput phenotyping of field-grown roots. We address this bottleneck with DIRT/3D, a newly developed image-based 3D root phenotyping platform, which measures 16 architecture traits in mature maize root systems. DIRT/3D computed reliably all 16 traits on a test panel of 12 contrasting maize genotypes. The analysis of the maize panel validated traits such as distance between whorls, and the number, angle, and diameters of crown and brace roots. Overall, we observed a coefficient of determination of r2>0.84 and a high broad-sense heritability of Embedded Image for all traits. Means of the 16 traits and a newly developed descriptor to characterize a complete root architecture distinguished all genotypes. DIRT/3D is a step towards automated quantification of highly occluded maize root systems in the field and will support breeders and root biologists to improved carbon sequestration and food security in the face of the adverse effects of climate change.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted July 02, 2020.
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DIRT/3D: 3D phenotyping for maize (Zea mays) root architecture in the field
Suxing Liu, Carlos Sherard Barrow, Meredith Hanlon, Jonathan P. Lynch, Alexander Bucksch
bioRxiv 2020.06.30.180059; doi: https://doi.org/10.1101/2020.06.30.180059
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DIRT/3D: 3D phenotyping for maize (Zea mays) root architecture in the field
Suxing Liu, Carlos Sherard Barrow, Meredith Hanlon, Jonathan P. Lynch, Alexander Bucksch
bioRxiv 2020.06.30.180059; doi: https://doi.org/10.1101/2020.06.30.180059

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