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Characterization of redox sensitive algal mannitol-1-phosphatases of the haloacid dehalogenase superfamily of proteins

Yoran Le Strat, View ORCID ProfileThierry Tonon, View ORCID ProfileCatherine Leblanc, View ORCID ProfileAgnès Groisillier
doi: https://doi.org/10.1101/2020.07.01.179531
Yoran Le Strat
aSorbonne Universités, UPMC Université Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France
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Thierry Tonon
bCentre for Novel Agricultural Products, Department of Biology, University of York, Heslington, York YO10 5DD, United Kingdom
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Catherine Leblanc
aSorbonne Universités, UPMC Université Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France
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Agnès Groisillier
aSorbonne Universités, UPMC Université Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France
cUnité Fonctionnalité et Ingénierie des Protéines (UFIP), UMR 6286 CNRS, Université de Nantes, 44322 Nantes, France
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  • For correspondence: agnes.groisillier@univ-nantes.fr
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Abstract

Macroalgae (or seaweeds) are the dominant primary producers in marine vegetated coastal habitats and largely contribute to global ocean carbon fluxes. They also represent attractive renewable production platforms for biofuels, food, feed, and bioactives, notably due to their diverse and peculiar polysaccharides and carbohydrates. Among seaweeds, brown algae produce alginates and sulfated fucans as constituents of their cell wall, and the photoassimilates laminarin and mannitol for carbon storage. Availability of brown algal genomes, including those of the kelp Saccharina japonica and the filamentous Ectocarpus sp., has paved the way for biochemical characterization of recombinant enzymes involved in their polysaccharide and carbohydrates synthesis, notably mannitol. Biosynthesis of mannitol in brown algae starts from fructose-6-phospate, which is converted into mannitol-1-phosphate (M1P), and this intermediate is then hydrolysed by a haloacid dehalogenase type M1P phosphatase (M1Pase) to produce mannitol. We report here the biochemical characterization of a second M1Pase in Ectocarpus sp after heterologous expression in Escherichia coli. (EsM1Pase1). Our results show that both Ectocarpus M1Pases were redox sensitive, with EsM1Pase1 being active only in presence of reducing agent. Such catalytic properties have not been observed for any of the M1Pase characterized so far. EsM1Pases were specific to mannitol, in contrast to S. japonica M1Pases that can use other phosphorylated sugars as substrates. Finally, brown algal M1Pases grouped into two well-supported clades, with potential different subcellular localization and physiological role(s) under diverse environmental conditions and/or stages of life cycle.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 01, 2020.
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Characterization of redox sensitive algal mannitol-1-phosphatases of the haloacid dehalogenase superfamily of proteins
Yoran Le Strat, Thierry Tonon, Catherine Leblanc, Agnès Groisillier
bioRxiv 2020.07.01.179531; doi: https://doi.org/10.1101/2020.07.01.179531
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Characterization of redox sensitive algal mannitol-1-phosphatases of the haloacid dehalogenase superfamily of proteins
Yoran Le Strat, Thierry Tonon, Catherine Leblanc, Agnès Groisillier
bioRxiv 2020.07.01.179531; doi: https://doi.org/10.1101/2020.07.01.179531

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