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Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives

Stephen R. Welch, Katherine A. Davies, Hubert Buczkowski, Nipunadi Hettiarachchi, Nicole Green, Ulrike Arnold, Matthew Jones, Matthew J. Hannah, Reah Evans, Christopher Burton, Jane E. Burton, Malcolm Guiver, Patricia A. Cane, Neil Woodford, Christine B. Bruce, Allen D. G. Roberts, Marian J. Killip
doi: https://doi.org/10.1101/2020.07.08.194613
Stephen R. Welch
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Katherine A. Davies
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Hubert Buczkowski
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Nipunadi Hettiarachchi
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Nicole Green
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Ulrike Arnold
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
bUK Public Health Rapid Support Team, Public Health England/London School of Hygiene and Tropical Medicine, London, UK
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Matthew Jones
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Matthew J. Hannah
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Reah Evans
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Christopher Burton
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Jane E. Burton
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Malcolm Guiver
cVirology Department, Clinical Sciences Laboratory, National Infection Service, Public Health England, Manchester Public Health Laboratory, Manchester, UK
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Patricia A. Cane
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Neil Woodford
dNIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Christine B. Bruce
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Allen D. G. Roberts
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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Marian J. Killip
aHigh Containment Microbiology, NIS Laboratories, National Infection Service, Public Health England, Colindale, London, UK
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  • For correspondence: marian.killip@phe.gov.uk
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Abstract

The COVID-19 pandemic has necessitated a rapid multi-faceted response by the scientific community, bringing researchers, health officials and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, the novel human coronavirus SARS-CoV-2. Work with infectious SARS-CoV-2 is currently restricted to high-containment laboratories, but material can be handled at a lower containment level after inactivation. Given the wide array of inactivation reagents that are being used in laboratories during this pandemic, it is vital that their effectiveness is thoroughly investigated. Here, we evaluated a total of 23 commercial reagents designed for clinical sample transportation, nucleic acid extraction and virus inactivation for their ability to inactivate SARS-CoV-2, as well as seven other common chemicals including detergents and fixatives. As part of this study, we have also tested five filtration matrices for their effectiveness at removing the cytotoxic elements of each reagent, permitting accurate determination of levels of infectious virus remaining following treatment. In addition to providing critical data informing inactivation methods and risk assessments for diagnostic and research laboratories working with SARS-CoV-2, these data provide a framework for other laboratories to validate their inactivation processes and to guide similar studies for other pathogens.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted July 10, 2020.
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Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives
Stephen R. Welch, Katherine A. Davies, Hubert Buczkowski, Nipunadi Hettiarachchi, Nicole Green, Ulrike Arnold, Matthew Jones, Matthew J. Hannah, Reah Evans, Christopher Burton, Jane E. Burton, Malcolm Guiver, Patricia A. Cane, Neil Woodford, Christine B. Bruce, Allen D. G. Roberts, Marian J. Killip
bioRxiv 2020.07.08.194613; doi: https://doi.org/10.1101/2020.07.08.194613
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Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives
Stephen R. Welch, Katherine A. Davies, Hubert Buczkowski, Nipunadi Hettiarachchi, Nicole Green, Ulrike Arnold, Matthew Jones, Matthew J. Hannah, Reah Evans, Christopher Burton, Jane E. Burton, Malcolm Guiver, Patricia A. Cane, Neil Woodford, Christine B. Bruce, Allen D. G. Roberts, Marian J. Killip
bioRxiv 2020.07.08.194613; doi: https://doi.org/10.1101/2020.07.08.194613

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