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Plate reader microrheology

Robert F. Hawkins, View ORCID ProfileGregg A. Duncan
doi: https://doi.org/10.1101/2020.07.10.197723
Robert F. Hawkins
1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742
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Gregg A. Duncan
1Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742
2Biophysics Program, University of Maryland, College Park, MD 20742
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  • ORCID record for Gregg A. Duncan
  • For correspondence: gaduncan@umd.edu
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Abstract

In this work, we report the development of a simplified microrheological method that can be used to rapidly study soft materials. This approach uses fluorescence polarization and a plate reader format to measure the rotational diffusion of nanoparticles within a sample of interest. We show that this measurement is sensitive to viscosity-dependent changes in polymeric soft materials and is correlated with particle tracking microrheology, a previously validated measure of microrheology. Using these fluorescence polarization-based measurements, we describe formalism that enables reasonable estimation of viscosity in polymeric materials after accounting for length-scale dependent effects of the polymer environment on the nanoparticle rotational diffusion. The use of a plate reader format allows this approach to be higher throughput, less technically challenging, and more widely accessible than standard macro- and microrheological methods, making it available to non-experts. This approach has potential applications in academic and industry settings where conventional rheological equipment may not be available, as well as in clinical settings to rapidly characterize human clinical samples.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 10, 2020.
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Plate reader microrheology
Robert F. Hawkins, Gregg A. Duncan
bioRxiv 2020.07.10.197723; doi: https://doi.org/10.1101/2020.07.10.197723
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Plate reader microrheology
Robert F. Hawkins, Gregg A. Duncan
bioRxiv 2020.07.10.197723; doi: https://doi.org/10.1101/2020.07.10.197723

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