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Friendly regulates membrane depolarization induced mitophagy in Arabidopsis

Juncai Ma, Zizhen Liang, Jierui Zhao, Pengfei Wang, Wenlong Ma, Juan A. Fernandez Andrade, Yonglun Zeng, Nenad Grujic, Liwen Jiang, View ORCID ProfileYasin Dagdas, View ORCID ProfileByung-Ho Kang
doi: https://doi.org/10.1101/2020.07.12.198424
Juncai Ma
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Zizhen Liang
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Jierui Zhao
2Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria
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Pengfei Wang
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Wenlong Ma
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Juan A. Fernandez Andrade
3Department of Physiology and Pharmacology, Western University, London, Ontario, Canada
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Yonglun Zeng
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Nenad Grujic
2Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria
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Liwen Jiang
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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Yasin Dagdas
2Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria
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  • ORCID record for Yasin Dagdas
Byung-Ho Kang
1School of Life Sciences, Centre for Cell & Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
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  • ORCID record for Byung-Ho Kang
  • For correspondence: bkang@cuhk.edu.hk
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Abstract

The oxidative environment within the mitochondria makes them particularly vulnerable to proteotoxic stress. To maintain a healthy mitochondrial network, eukaryotes have evolved multi-tiered quality control pathways. If the stress cannot be alleviated, defective mitochondria are selectively removed by autophagy via a process termed mitophagy. Despite significant advances in metazoans and yeast, in plants, the molecular underpinnings of mitophagy are largely unknown. Here, using time-lapse imaging, electron tomography and biochemical assays, we show that uncoupler treatments cause loss of mitochondrial membrane potential and induce autophagy in Arabidopsis. The damaged mitochondria are selectively engulfed by autophagosomes that are ATG5 dependent and labelled by ATG8 proteins. Friendly, a member of the Clustered Mitochondria protein family, is recruited to the damaged mitochondria to mediate mitophagy. In addition to stress, mitophagy is also induced during de-etiolation, a major cellular transformation during photomorphogenesis that involves chloroplast biogenesis. De-etiolation triggered mitophagy regulates cotyledon greening, pointing towards an inter-organellar cross-talk mechanism. Altogether our results demonstrate how plants employ mitophagy to recycle damaged mitochondria during stress and development.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* e-mail: bkang{at}cuhk.edu.hk, yasin.dagdas{at}gmi.oeaw.ac.at

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 12, 2020.
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Friendly regulates membrane depolarization induced mitophagy in Arabidopsis
Juncai Ma, Zizhen Liang, Jierui Zhao, Pengfei Wang, Wenlong Ma, Juan A. Fernandez Andrade, Yonglun Zeng, Nenad Grujic, Liwen Jiang, Yasin Dagdas, Byung-Ho Kang
bioRxiv 2020.07.12.198424; doi: https://doi.org/10.1101/2020.07.12.198424
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Friendly regulates membrane depolarization induced mitophagy in Arabidopsis
Juncai Ma, Zizhen Liang, Jierui Zhao, Pengfei Wang, Wenlong Ma, Juan A. Fernandez Andrade, Yonglun Zeng, Nenad Grujic, Liwen Jiang, Yasin Dagdas, Byung-Ho Kang
bioRxiv 2020.07.12.198424; doi: https://doi.org/10.1101/2020.07.12.198424

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