Abstract
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible “up” or receptor-inaccessible “down” conformations. Here, we report that the commonly used stabilized S ectodomain construct “2P” is sensitive to cold temperature, and that this cold sensitivity is resolved in a “down” state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
1. Figures have been re-organized. 2. New data showing characterization of two antibodies isolated from convalescent COVID-19 patients added. 3. Methods updated.
Copyright
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