Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Roles of motor on-rate and cargo mobility in intracellular transport

Matthew J. Bovyn, Babu J.N. Reddy, Steven P. Gross, Jun F. Allard
doi: https://doi.org/10.1101/2020.07.13.201434
Matthew J. Bovyn
aDepartment of Physics and Astronomy, University of California, Irvine
1Center for Complex Biological Systems, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Babu J.N. Reddy
bDepartment of Developmental and Cell Biology, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steven P. Gross
bDepartment of Developmental and Cell Biology, University of California, Irvine
aDepartment of Physics and Astronomy, University of California, Irvine
cDepartment of Biomedical Engineering, University of California, Irvine
1Center for Complex Biological Systems, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sgross@uci.edu jun.allard@uci.edu
Jun F. Allard
aDepartment of Physics and Astronomy, University of California, Irvine
dDepartment of Mathematics, University of California, Irvine
1Center for Complex Biological Systems, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sgross@uci.edu jun.allard@uci.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Molecular motors like kinesin are critical for cellular organization and biological function including in neurons. There is detailed understanding of how they move and how factors such as applied force and the presence of microtubule-associated proteins can alter this single-motor travel. In order to walk, the cargo-motor complex must first attach to a microtubule. This attachment process is less studied. Here, we use a combination of single-molecule bead experiments, modeling, and simulation to examine how cargos with kinesin-1 bind to microtubules. In experiment, we find that increasing cargo size and environment viscosity both signficantly slow cargo binding time. We use modeling and simulation to examine how the single motor on rate translates to the on rate of the cargo. Combining experiment and modeling allows us to estimate the single motor on rate as 100 s−1. This is a much higher value than previous estimates. We attribute the difference between our measurements and previous estimates to two factors: first, we are directly measuring initial motor attachment (as opposed to re-binding of a second motor) and second, the theoretical framework allows us to account for missed events (i.e. binding events not detected by the experiments due to their short duration). This indicates that the mobility of the cargo itself, determined by its size and interaction with the cytoplasmic environment, play a previously underestimated role in determining intracellular transport kinetics.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted July 14, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Roles of motor on-rate and cargo mobility in intracellular transport
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Roles of motor on-rate and cargo mobility in intracellular transport
Matthew J. Bovyn, Babu J.N. Reddy, Steven P. Gross, Jun F. Allard
bioRxiv 2020.07.13.201434; doi: https://doi.org/10.1101/2020.07.13.201434
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Roles of motor on-rate and cargo mobility in intracellular transport
Matthew J. Bovyn, Babu J.N. Reddy, Steven P. Gross, Jun F. Allard
bioRxiv 2020.07.13.201434; doi: https://doi.org/10.1101/2020.07.13.201434

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4683)
  • Biochemistry (10360)
  • Bioengineering (7675)
  • Bioinformatics (26336)
  • Biophysics (13528)
  • Cancer Biology (10686)
  • Cell Biology (15440)
  • Clinical Trials (138)
  • Developmental Biology (8497)
  • Ecology (12821)
  • Epidemiology (2067)
  • Evolutionary Biology (16857)
  • Genetics (11399)
  • Genomics (15478)
  • Immunology (10617)
  • Microbiology (25217)
  • Molecular Biology (10223)
  • Neuroscience (54468)
  • Paleontology (401)
  • Pathology (1668)
  • Pharmacology and Toxicology (2897)
  • Physiology (4342)
  • Plant Biology (9247)
  • Scientific Communication and Education (1586)
  • Synthetic Biology (2558)
  • Systems Biology (6781)
  • Zoology (1466)