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Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo

Yi Xie, View ORCID ProfileJ. Todd Blankenship
doi: https://doi.org/10.1101/2020.07.14.200857
Yi Xie
1Department of Biological Sciences, University of Denver, Denver, CO 80208, USA
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J. Todd Blankenship
1Department of Biological Sciences, University of Denver, Denver, CO 80208, USA
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  • ORCID record for J. Todd Blankenship
  • For correspondence: todd.blankenship@du.edu
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SUMMARY

Despite extensive studies on the actin regulators that direct microfilament dynamics, how these regulators are combinatorially utilized in organismal tissues to generate 3D structures is an unresolved question. Here, we present an in-depth characterization of cortical actin cap dynamics and their regulation in vivo. We identify rapid phases of initiation, expansion, duplication and disassembly and examine the functions of 7 different Actin and/or Nucleator Regulators (ANRPs) in guiding these behaviors. We find ANRPs provide distinct but cooperative activities in building actin cap morphologies – specifically, while DPod1 is a major regulator of actin intensities, Cortactin is required for continued cortical growth, while Coronin functions in both growth and intensity and is required for Cortactin localization to the cap periphery. Unexpectedly, cortical actin populations recover more rapidly after regulator disruption, suggestive of a potential deep competition for limited G-actin pools, and we measure in vivo Arp2/3 recruitment efficiencies through an ectopic relocalization strategy. Our results illustrate how the coordination of multiple actin regulators can orchestrate organized and dynamic actin structures in an in vivo system.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Lead contact: Todd Blankenship

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 14, 2020.
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Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo
Yi Xie, J. Todd Blankenship
bioRxiv 2020.07.14.200857; doi: https://doi.org/10.1101/2020.07.14.200857
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Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivo
Yi Xie, J. Todd Blankenship
bioRxiv 2020.07.14.200857; doi: https://doi.org/10.1101/2020.07.14.200857

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