Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution

Keisuke Ota, Yasuhiro Oisi, Takayuki Suzuki, Muneki Ikeda, Yoshiki Ito, Tsubasa Ito, Kenta Kobayashi, Midori Kobayashi, Maya Odagawa, Chie Matsubara, Yoshinori Kuroiwa, Masaru Horikoshi, Junya Matsushita, Hiroyuki Hioki, Masamichi Ohkura, Junichi Nakai, Masafumi Oizumi, Atsushi Miyawaki, View ORCID ProfileToru Aonishi, Takahiro Ode, View ORCID ProfileMasanori Murayama
doi: https://doi.org/10.1101/2020.07.14.201699
Keisuke Ota
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuhiro Oisi
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takayuki Suzuki
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Muneki Ikeda
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
2Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
3Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshiki Ito
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
4Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsubasa Ito
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
5School of Computing, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kenta Kobayashi
6Section of Viral Vector Development, National Institute for Physiological Sciences, 38 Nishigonaka, Myodaiji-cho, Okazaki-shi, Aichi 444-8585, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Midori Kobayashi
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maya Odagawa
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chie Matsubara
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshinori Kuroiwa
7Designing Department, Technology Solutions Sector, Healthcare Business Unit, Nikon Corporation, 471 Nagaodai-cho, Sakae-ku, Yokohama, Kanagawa 244-8533, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masaru Horikoshi
7Designing Department, Technology Solutions Sector, Healthcare Business Unit, Nikon Corporation, 471 Nagaodai-cho, Sakae-ku, Yokohama, Kanagawa 244-8533, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Junya Matsushita
8Application Engineer, Business Promotion Group No. 1, Electron Tube Division, Hamamatsu Photonics K.K., 314-5 Shimokanzo, Iwata-shi, Shizuoka 438-0193, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroyuki Hioki
9Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masamichi Ohkura
10Department of Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshinomachi, Nobeoka-shi, Miyazaki 882-8508, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Junichi Nakai
11Graduate School of Dentistry, Tohoku University, 4-1 Seiryou-machi, Aoba-ku, Sendai-shi, Miyagi 980-8575, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masafumi Oizumi
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
2Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Atsushi Miyawaki
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toru Aonishi
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
5School of Computing, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Toru Aonishi
Takahiro Ode
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
12FOV Corporation, 2-12-3 Taru-machi, Kouhoku-ku, Yokohama, Kanagawa 222-0001, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masanori Murayama
1Center for Brain Science, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Masanori Murayama
  • For correspondence: masanori.murayama@riken.jp
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Fast and wide imaging with single-cell resolution, high signal-to-noise ratio and no optical aberration has the potential to open up new avenues of investigation in biology. However, this imaging is challenging because of the inevitable tradeoffs among those parameters. Here, we overcome the tradeoffs by combining a resonant scanning system, a large objective with low magnification and high numerical aperture, and highly sensitive large-aperture photodetectors. The result is a practically aberration-free, fast scanning high optical invariant two-photon microscopy (FASHIO-2PM) that enables calcium imaging from a large network composed of ∼16k neurons at 7.5 Hz in a 9 mm2 contiguous image plane including more than 10 sensory-motor and higher-order regions of the cerebral cortex in awake mice. Through a network analysis based on single-cell activities, we discover that the brain exhibits small-world-ness rather than scale-freeness. FASHIO-2PM will enable revealing biological dynamics by simultaneous monitoring of macroscopic activity and its composing elements.

Competing Interest Statement

Takahiro Ode is a founder of FOV Corporation. Junya Matsushita is an employee of Hamamatsu Photonics K.K. Yoshinori Kuroiwa and Masaru Horikoshi are employees of Nikon Corporation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 15, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution
Keisuke Ota, Yasuhiro Oisi, Takayuki Suzuki, Muneki Ikeda, Yoshiki Ito, Tsubasa Ito, Kenta Kobayashi, Midori Kobayashi, Maya Odagawa, Chie Matsubara, Yoshinori Kuroiwa, Masaru Horikoshi, Junya Matsushita, Hiroyuki Hioki, Masamichi Ohkura, Junichi Nakai, Masafumi Oizumi, Atsushi Miyawaki, Toru Aonishi, Takahiro Ode, Masanori Murayama
bioRxiv 2020.07.14.201699; doi: https://doi.org/10.1101/2020.07.14.201699
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution
Keisuke Ota, Yasuhiro Oisi, Takayuki Suzuki, Muneki Ikeda, Yoshiki Ito, Tsubasa Ito, Kenta Kobayashi, Midori Kobayashi, Maya Odagawa, Chie Matsubara, Yoshinori Kuroiwa, Masaru Horikoshi, Junya Matsushita, Hiroyuki Hioki, Masamichi Ohkura, Junichi Nakai, Masafumi Oizumi, Atsushi Miyawaki, Toru Aonishi, Takahiro Ode, Masanori Murayama
bioRxiv 2020.07.14.201699; doi: https://doi.org/10.1101/2020.07.14.201699

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4399)
  • Biochemistry (9637)
  • Bioengineering (7127)
  • Bioinformatics (24958)
  • Biophysics (12676)
  • Cancer Biology (10002)
  • Cell Biology (14406)
  • Clinical Trials (138)
  • Developmental Biology (7991)
  • Ecology (12152)
  • Epidemiology (2067)
  • Evolutionary Biology (16029)
  • Genetics (10956)
  • Genomics (14784)
  • Immunology (9911)
  • Microbiology (23750)
  • Molecular Biology (9516)
  • Neuroscience (51101)
  • Paleontology (370)
  • Pathology (1545)
  • Pharmacology and Toxicology (2694)
  • Physiology (4038)
  • Plant Biology (8700)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2406)
  • Systems Biology (6461)
  • Zoology (1350)