Abstract
We report BiPOLES, an optogenetic tool for balanced excitation and inhibition of neurons with light of two different colors. BiPOLES consists of the blue-light-sensitive anion-conducting channelrhodopsin GtACR2 fused to the red-light-sensitive cation-conducting channelrhodopsin Chrimson in a single, trafficking-optimized tandem protein. BiPOLES enables multiple applications including potent dual-color spiking and silencing of the same neurons in vivo and dual-color optogenetic control of two independent neuronal populations.
Competing Interest Statement
The authors have declared no competing interest.
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