Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Distinct signatures of calcium activity in brain pericytes

View ORCID ProfileChaim Glück, View ORCID ProfileKim David Ferrari, Annika Keller, Aiman S. Saab, Jillian L. Stobart, View ORCID ProfileBruno Weber
doi: https://doi.org/10.1101/2020.07.16.207076
Chaim Glück
1Experimental Imaging and Neuroenergetics, Institute of Pharmacology and Toxicology, University of Zürich, Switzerland
2Neuroscience Center Zürich, ETH Zürich and University of Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Chaim Glück
Kim David Ferrari
1Experimental Imaging and Neuroenergetics, Institute of Pharmacology and Toxicology, University of Zürich, Switzerland
2Neuroscience Center Zürich, ETH Zürich and University of Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kim David Ferrari
Annika Keller
2Neuroscience Center Zürich, ETH Zürich and University of Zürich, Switzerland
3Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zürich, University of Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aiman S. Saab
1Experimental Imaging and Neuroenergetics, Institute of Pharmacology and Toxicology, University of Zürich, Switzerland
2Neuroscience Center Zürich, ETH Zürich and University of Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jillian L. Stobart
1Experimental Imaging and Neuroenergetics, Institute of Pharmacology and Toxicology, University of Zürich, Switzerland
4College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bruno Weber
1Experimental Imaging and Neuroenergetics, Institute of Pharmacology and Toxicology, University of Zürich, Switzerland
2Neuroscience Center Zürich, ETH Zürich and University of Zürich, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bruno Weber
  • For correspondence: bweber@pharma.uzh.ch
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Even though pericytes have been implicated in various neurological disorders, little is known about their function and signaling pathways in the healthy brain. Here, we characterized cortical pericyte calcium dynamics using two-photon imaging of Pdgfrβ-CreERT2;GCaMP6s mice under anesthesia in vivo and in brain slices ex vivo. We found distinct differences between pericyte subtypes in vivo: Ensheathing pericytes exhibited smooth muscle cell-like calcium dynamics, while calcium signals in capillary pericytes were irregular, higher in frequency and occurred in cellular microdomains. In contrast to ensheathing pericytes, capillary pericytes retained their spontaneous calcium signals during prolonged anesthesia and in the absence of blood flow ex vivo. Chemogenetic activation of neurons in vivo and acute increase of extracellular potassium in brain slices strongly decreased calcium activity in capillary pericytes. We propose that neuronal activity-induced elevations in extracellular potassium suppress calcium activity in capillary pericytes, likely mediated by Kir2.2 and KATP channel activation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted July 18, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Distinct signatures of calcium activity in brain pericytes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Distinct signatures of calcium activity in brain pericytes
Chaim Glück, Kim David Ferrari, Annika Keller, Aiman S. Saab, Jillian L. Stobart, Bruno Weber
bioRxiv 2020.07.16.207076; doi: https://doi.org/10.1101/2020.07.16.207076
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Distinct signatures of calcium activity in brain pericytes
Chaim Glück, Kim David Ferrari, Annika Keller, Aiman S. Saab, Jillian L. Stobart, Bruno Weber
bioRxiv 2020.07.16.207076; doi: https://doi.org/10.1101/2020.07.16.207076

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (2430)
  • Biochemistry (4791)
  • Bioengineering (3333)
  • Bioinformatics (14684)
  • Biophysics (6640)
  • Cancer Biology (5172)
  • Cell Biology (7429)
  • Clinical Trials (138)
  • Developmental Biology (4367)
  • Ecology (6874)
  • Epidemiology (2057)
  • Evolutionary Biology (9926)
  • Genetics (7346)
  • Genomics (9533)
  • Immunology (4558)
  • Microbiology (12686)
  • Molecular Biology (4948)
  • Neuroscience (28348)
  • Paleontology (199)
  • Pathology (809)
  • Pharmacology and Toxicology (1392)
  • Physiology (2024)
  • Plant Biology (4504)
  • Scientific Communication and Education (977)
  • Synthetic Biology (1299)
  • Systems Biology (3917)
  • Zoology (726)