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SARS-CoV-2 RNA shedding in recovered COVID-19 cases and the presence of antibodies against SARS-CoV-2 in recovered COVID-19 cases and close contacts

Chintana Chirathaworn, Manit Sripramote, Piti Chalongviriyalert, Supunee Jirajariyavej, Phatharaporn Kiatpanabhikul, Jatuporn Saiyarin, Chulikorn Soudon, Orawan Thienfaidee, Thitisan Palakawong Na Ayuthaya, Chantapat Brukesawan, Dootchai Chaiwanichsiri, Duangnapa Intharasongkroh, Nasamon Wanlapakorn, Jira Chansaenroj, Jiratchaya Puenpa, View ORCID ProfileRitthideach Yorsaeng, Arunee Thitithanyanont, Rungrueng Kitphati, Anek Mungaomklang, Pijaya Nagavajara, View ORCID ProfileYong Poovorawan
doi: https://doi.org/10.1101/2020.07.17.208439
Chintana Chirathaworn
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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Manit Sripramote
2Medical Service Department, Bangkok Metropolitan Administration
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Piti Chalongviriyalert
2Medical Service Department, Bangkok Metropolitan Administration
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Supunee Jirajariyavej
3Taksin Hospital, Medical Service Department, Bangkok Metropolitan Administration
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Phatharaporn Kiatpanabhikul
4Charoenkrung Pracharak Hospital, Medical Service Department, Bangkok Metropolitan Administration
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Jatuporn Saiyarin
5Klang General Hospital, Medical Service Department, Bangkok Metropolitan Administration
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Chulikorn Soudon
6Sirindhorn Hospital, Medical Service Department, Bangkok Metropolitan Administration
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Orawan Thienfaidee
7Ratchaphiphat Hospital, Medical service department, Bangkok Metropolitan Administration
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Thitisan Palakawong Na Ayuthaya
8Public Health Center 28, Health department, Bangkok Metropolitan Administration
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Chantapat Brukesawan
9Public Health Center 26, Health department, Bangkok Metropolitan Administration
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Dootchai Chaiwanichsiri
10National Blood Center, Thai Red Cross Society
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Duangnapa Intharasongkroh
10National Blood Center, Thai Red Cross Society
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Nasamon Wanlapakorn
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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Jira Chansaenroj
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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Jiratchaya Puenpa
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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Ritthideach Yorsaeng
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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Arunee Thitithanyanont
11Department of Microbiology, Faculty of Science, Mahidol University
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Rungrueng Kitphati
12Institute for Urban Disease Control and Prevention, Department of Disease Control, Ministry of Public Health, Bangkok, Thailand
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Anek Mungaomklang
12Institute for Urban Disease Control and Prevention, Department of Disease Control, Ministry of Public Health, Bangkok, Thailand
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Pijaya Nagavajara
13Office of the Permanent Secretary for the Bangkok Metropolitan Administration
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Yong Poovorawan
1Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand
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  • For correspondence: yong.p@chula.ac.th
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Abstract

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). COVID-19 emerged in December 2019 and has spread globally. Although Thailand has been effective at controlling the spread of COVID-19, disease surveillance and information on antibody responses in infected cases and close contacts are needed because there is still no specific treatment or vaccine available. We investigated 217 recovered COVID-19 cases to monitor their viral RNA shedding and production of antibodies against SARS-CoV-2. The presence of antibodies in blood samples from 308 close contacts of COVID-19 cases was also determined. Viral RNA was still detectable in 6.6 % of recovered COVID-19 cases. The most prolonged duration of viral RNA shedding detected in this study was 105 days. IgM, IgG, and IgA antibodies against SARS-CoV-2 were detected in 13.82, 88.48, and 83.41 % of the recovered cases 4–12 weeks after disease onset, respectively. Although the patients had recovered from their illness, the levels of antibodies detected showed association with their symptoms during their stay in hospital. Fifteen of the 308 contacts (4.87 %) of COVID-19 cases tested positive for IgG antibodies. The presence of antibodies against SARS-CoV-2 suggested that there was viral exposure among close contacts. Viral clearance and the pattern of antibody responses in infected individuals are both crucial for effectively combatting SARS-CoV-2. Our study provides additional information on the natural history of this newly emerging disease related to both natural host defenses and a strategy for vaccine development.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted July 17, 2020.
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SARS-CoV-2 RNA shedding in recovered COVID-19 cases and the presence of antibodies against SARS-CoV-2 in recovered COVID-19 cases and close contacts
Chintana Chirathaworn, Manit Sripramote, Piti Chalongviriyalert, Supunee Jirajariyavej, Phatharaporn Kiatpanabhikul, Jatuporn Saiyarin, Chulikorn Soudon, Orawan Thienfaidee, Thitisan Palakawong Na Ayuthaya, Chantapat Brukesawan, Dootchai Chaiwanichsiri, Duangnapa Intharasongkroh, Nasamon Wanlapakorn, Jira Chansaenroj, Jiratchaya Puenpa, Ritthideach Yorsaeng, Arunee Thitithanyanont, Rungrueng Kitphati, Anek Mungaomklang, Pijaya Nagavajara, Yong Poovorawan
bioRxiv 2020.07.17.208439; doi: https://doi.org/10.1101/2020.07.17.208439
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SARS-CoV-2 RNA shedding in recovered COVID-19 cases and the presence of antibodies against SARS-CoV-2 in recovered COVID-19 cases and close contacts
Chintana Chirathaworn, Manit Sripramote, Piti Chalongviriyalert, Supunee Jirajariyavej, Phatharaporn Kiatpanabhikul, Jatuporn Saiyarin, Chulikorn Soudon, Orawan Thienfaidee, Thitisan Palakawong Na Ayuthaya, Chantapat Brukesawan, Dootchai Chaiwanichsiri, Duangnapa Intharasongkroh, Nasamon Wanlapakorn, Jira Chansaenroj, Jiratchaya Puenpa, Ritthideach Yorsaeng, Arunee Thitithanyanont, Rungrueng Kitphati, Anek Mungaomklang, Pijaya Nagavajara, Yong Poovorawan
bioRxiv 2020.07.17.208439; doi: https://doi.org/10.1101/2020.07.17.208439

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