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Variability of an early developmental cell population underlies stochastic laterality defects

View ORCID ProfileRoberto Moreno-Ayala, Pedro Olivares-Chauvet, Ronny Schäfer, View ORCID ProfileJan Philipp Junker
doi: https://doi.org/10.1101/2020.07.20.212282
Roberto Moreno-Ayala
1Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, Berlin, 10115, Germany
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  • ORCID record for Roberto Moreno-Ayala
Pedro Olivares-Chauvet
1Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, Berlin, 10115, Germany
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Ronny Schäfer
1Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, Berlin, 10115, Germany
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Jan Philipp Junker
1Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, Berlin, 10115, Germany
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  • ORCID record for Jan Philipp Junker
  • For correspondence: janphilipp.junker@mdc-berlin.de
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Summary

Embryonic development seemingly proceeds with almost perfect precision. However, it is largely unknown how much underlying microscopic variability is compatible with normal development. Here, we quantified embryo-to-embryo variability in vertebrate development, by studying cell number variation in the zebrafish endoderm. We noticed that the size of a sub-population of the endoderm, the dorsal forerunner cells (which later form the left-right organizer), exhibits significantly more embryo-to-embryo variation than the rest of the endoderm. We found that, when incubated at elevated temperature, the frequency of left-right laterality defects is increased drastically in embryos with a low number of dorsal forerunner cells. Furthermore, we observed that these fluctuations have a large stochastic component among fish of the same genetic background. Hence, a stochastic variation in early development leads to a remarkably strong macroscopic phenotype. These fluctuations appear to be associated with maternal effects in the specification of the dorsal forerunner cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE153621

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 08, 2020.
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Variability of an early developmental cell population underlies stochastic laterality defects
Roberto Moreno-Ayala, Pedro Olivares-Chauvet, Ronny Schäfer, Jan Philipp Junker
bioRxiv 2020.07.20.212282; doi: https://doi.org/10.1101/2020.07.20.212282
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Variability of an early developmental cell population underlies stochastic laterality defects
Roberto Moreno-Ayala, Pedro Olivares-Chauvet, Ronny Schäfer, Jan Philipp Junker
bioRxiv 2020.07.20.212282; doi: https://doi.org/10.1101/2020.07.20.212282

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