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Differential activation of JAK-STAT signaling in blood cell progenitors reveals functional compartmentalization of the Drosophila lymph gland

Diana Rodrigues, Yoan Renaud, K. VijayRaghavan, Lucas Waltzer, Maneesha S. Inamdar
doi: https://doi.org/10.1101/2020.07.26.219717
Diana Rodrigues
1Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
2National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
3Shanmugha Arts, Science, Technology & Research Academy, Tamil Nadu, India
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Yoan Renaud
4University of Clermont Auvergne, CNRS, Inserm, GReD, Clermont-Ferrand, France
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K. VijayRaghavan
2National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
3Shanmugha Arts, Science, Technology & Research Academy, Tamil Nadu, India
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Lucas Waltzer
4University of Clermont Auvergne, CNRS, Inserm, GReD, Clermont-Ferrand, France
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  • For correspondence: inamdar@jncasr.ac.in lucas.waltzer@uca.fr
Maneesha S. Inamdar
1Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
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  • For correspondence: inamdar@jncasr.ac.in lucas.waltzer@uca.fr
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Abstract

Blood cells arise from diverse pools of stem and progenitor cells. Understanding progenitor heterogeneity is a major challenge. The Drosophila larval lymph gland is a well-studied model to understand blood progenitor maintenance and recapitulates several aspects of vertebrate hematopoiesis. However in-depth analysis has focused on progenitors located in lymph gland anterior lobes (AP), ignoring the progenitors from the posterior lobes (PP). Using in situ expression mapping and transcriptome analysis we reveal PP heterogeneity and identify molecular-genetic tools to study this abundant progenitor population. Functional analysis shows that PP resist differentiation upon immune challenge, in a JAK-STAT-dependent manner. Upon wasp parasitism, AP downregulate JAK-STAT signaling and form lamellocytes. In contrast, we show that PP activate STAT92E and remain undifferentiated. Stat92E knockdown in PP or genetically reducing JAK-STAT signaling permits PP lamellocyte differentiation. We discuss how heterogeneity and compartmentalization allow functional segregation in response to systemic cues and could be widely applicable.

Highlights We provide an in situ and transcriptome map of larval blood progenitors Posterior lymph gland progenitors are refractory to immune challenge STAT activation after wasp parasitism maintains posterior progenitors

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 26, 2020.
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Differential activation of JAK-STAT signaling in blood cell progenitors reveals functional compartmentalization of the Drosophila lymph gland
Diana Rodrigues, Yoan Renaud, K. VijayRaghavan, Lucas Waltzer, Maneesha S. Inamdar
bioRxiv 2020.07.26.219717; doi: https://doi.org/10.1101/2020.07.26.219717
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Differential activation of JAK-STAT signaling in blood cell progenitors reveals functional compartmentalization of the Drosophila lymph gland
Diana Rodrigues, Yoan Renaud, K. VijayRaghavan, Lucas Waltzer, Maneesha S. Inamdar
bioRxiv 2020.07.26.219717; doi: https://doi.org/10.1101/2020.07.26.219717

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