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Dehydrated Caenorhabditis elegans stocks are resistant to multiple freeze-thaw cycles

Patrick D. McClanahan, Richard J. McCloskey, Melanie N. T. Hing, David M. Raizen, Christopher Fang-Yen
doi: https://doi.org/10.1101/2020.07.27.223560
Patrick D. McClanahan
*Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania
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Richard J. McCloskey
*Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania
†Department of Neurology, Perelman School of Medicine, University of Pennsylvania
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Melanie N. T. Hing
*Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania
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David M. Raizen
†Department of Neurology, Perelman School of Medicine, University of Pennsylvania
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Christopher Fang-Yen
*Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania
‡Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania
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  • For correspondence: fangyen@seas.upenn.edu
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ABSTRACT

Ultracold preservation is widely used for storage of genetic stocks of Caenorhabditis elegans. Current cryopreservation protocols are vulnerable to refrigeration failures, which can result in the loss of stock viability due to damage during re-freezing. Here we present a method for preserving worms in a dehydrated and frozen form that retains viability after multiple freeze/thaw cycles. After dehydration in the presence of trehalose or glycerol, C. elegans stocks can be frozen and thawed multiple times while maintaining viability. Both stress-resistant dauer and non-dauer larvae survive desiccation and freezing. Survival after desiccation is dependent on the forkhead box transcription factor homologue DAF-16. Our technique is useful for storing stocks in a manner robust to freezer failures, and potentially for shipping strains between laboratories.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 29, 2020.
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Dehydrated Caenorhabditis elegans stocks are resistant to multiple freeze-thaw cycles
Patrick D. McClanahan, Richard J. McCloskey, Melanie N. T. Hing, David M. Raizen, Christopher Fang-Yen
bioRxiv 2020.07.27.223560; doi: https://doi.org/10.1101/2020.07.27.223560
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Dehydrated Caenorhabditis elegans stocks are resistant to multiple freeze-thaw cycles
Patrick D. McClanahan, Richard J. McCloskey, Melanie N. T. Hing, David M. Raizen, Christopher Fang-Yen
bioRxiv 2020.07.27.223560; doi: https://doi.org/10.1101/2020.07.27.223560

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