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CelLEVITAS: Label-free rapid sorting and enrichment of live cells via magnetic levitation

Elliot K. Chin, Colin A. Grant, Mehmet Giray Ogut, Bocheng Cai, Naside Gozde Durmus
doi: https://doi.org/10.1101/2020.07.27.223917
Elliot K. Chin
1Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Palo Alto, CA 94304, USA
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Colin A. Grant
1Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Palo Alto, CA 94304, USA
2Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA
3School of Engineering, Stanford University, Stanford, CA 94305, USA
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Mehmet Giray Ogut
2Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA
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Bocheng Cai
1Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Palo Alto, CA 94304, USA
4School of Public Health, University of California, Berkeley, Berkeley, CA 94270, USA
5College of Letters & Science, University of California, Berkeley, Berkeley, CA 94270, USA
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Naside Gozde Durmus
1Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Palo Alto, CA 94304, USA
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  • For correspondence: gdurmus@stanford.edu
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Abstract

Sorting methods that remove non-viable cells and debris, while retaining a high yield of viable cells, are crucial for many applications in biotechnology, genomics, tissue engineering and medicine. However, a significant challenge is gentle sorting of these different cell states based on very minute differences in density and magnetic signatures, without relying on any labels, tags or markers. Here, a new magnetic levitation-based technology, CelLEVITAS, is developed for the label-free sorting and enrichment of live cells. This work reports the first use of magnetic levitation for sorting of viable and non-viable cells within a microfluidic device and demonstrates extremely effective removal of dead cells and debris from heterogeneous samples. First, the levitation conditions for separating viable and non-viable cells under a magnetic field were fine-tuned. Levitation trajectories of live and dead cell states were then monitored in real-time, as cells magnetically focused to their corresponding levitation bands. CelLEVITAS successfully sorted and enriched live cells from a variety of input cell concentrations (100-200,000 cells/mL) and a variety of input purities (10-50%) into consistently high output purities (>80%). This method is sensitive, does not impair cell viability during sorting, and significantly increases the input sample viability up to 7-fold. Overall, this new magnetic levitation-based sorting strategy drastically reduces the processing time to a single-step, 30-minute sorting protocol and eliminates the manual pre-processing and labeling steps that are required for traditional flow cytometry techniques.

Competing Interest Statement

N.G.D is a co-founder of and has an equity interest in Levitas Bio, Inc., a company that develops new biotechnology tools for cell sorting and diagnostics. Her interests were viewed and managed in accordance with the conflict of interest policies.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 29, 2020.
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CelLEVITAS: Label-free rapid sorting and enrichment of live cells via magnetic levitation
Elliot K. Chin, Colin A. Grant, Mehmet Giray Ogut, Bocheng Cai, Naside Gozde Durmus
bioRxiv 2020.07.27.223917; doi: https://doi.org/10.1101/2020.07.27.223917
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CelLEVITAS: Label-free rapid sorting and enrichment of live cells via magnetic levitation
Elliot K. Chin, Colin A. Grant, Mehmet Giray Ogut, Bocheng Cai, Naside Gozde Durmus
bioRxiv 2020.07.27.223917; doi: https://doi.org/10.1101/2020.07.27.223917

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