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Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism

View ORCID ProfileFadi Jacob, Sarshan R. Pather, Wei-Kai Huang, Samuel Zheng Hao Wong, Haowen Zhou, Feng Zhang, Beatrice Cubitt, Catherine Z. Chen, Miao Xu, Manisha Pradhan, Daniel Y. Zhang, Wei Zheng, Anne G. Bang, Hongjun Song, Juan Carlos de a Torre, Guo-li Ming
doi: https://doi.org/10.1101/2020.07.28.225151
Fadi Jacob
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
3Medical Scientist Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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  • ORCID record for Fadi Jacob
Sarshan R. Pather
4Cell and Molecular Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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Wei-Kai Huang
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
5Graduate Program in Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Samuel Zheng Hao Wong
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
6Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Haowen Zhou
7Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
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Feng Zhang
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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Beatrice Cubitt
8Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA
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Catherine Z. Chen
9National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA
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Miao Xu
9National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA
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Manisha Pradhan
9National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA
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Daniel Y. Zhang
10Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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Wei Zheng
9National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA
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Anne G. Bang
7Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
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  • For correspondence: abang@sbpdiscovery.org shongjun@pennmedicine.upenn.edu juanct@scripps.edu gming@pennmedicine.upenn.edu
Hongjun Song
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
11Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
12Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
13The Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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  • For correspondence: abang@sbpdiscovery.org shongjun@pennmedicine.upenn.edu juanct@scripps.edu gming@pennmedicine.upenn.edu
Juan Carlos de a Torre
8Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA
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  • For correspondence: abang@sbpdiscovery.org shongjun@pennmedicine.upenn.edu juanct@scripps.edu gming@pennmedicine.upenn.edu
Guo-li Ming
1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
11Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
12Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
14Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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  • For correspondence: abang@sbpdiscovery.org shongjun@pennmedicine.upenn.edu juanct@scripps.edu gming@pennmedicine.upenn.edu
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Article Information

doi 
https://doi.org/10.1101/2020.07.28.225151
History 
  • July 28, 2020.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Fadi Jacob1,2,3,
  2. Sarshan R. Pather4,
  3. Wei-Kai Huang1,5,
  4. Samuel Zheng Hao Wong1,6,
  5. Haowen Zhou7,
  6. Feng Zhang1,
  7. Beatrice Cubitt8,
  8. Catherine Z. Chen9,
  9. Miao Xu9,
  10. Manisha Pradhan9,
  11. Daniel Y. Zhang10,
  12. Wei Zheng9,
  13. Anne G. Bang7,*,
  14. Hongjun Song1,11,12,13,*,
  15. Juan Carlos de a Torre8,* and
  16. Guo-li Ming1,11,12,14,15,*
  1. 1Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  2. 2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
  3. 3Medical Scientist Training Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
  4. 4Cell and Molecular Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  5. 5Graduate Program in Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
  6. 6Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
  7. 7Conrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
  8. 8Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA
  9. 9National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA
  10. 10Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  11. 11Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  12. 12Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  13. 13The Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  14. 14Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
  1. ↵*Correspondence: Anne G. Bang (abang{at}sbpdiscovery.org), Hongjun Song (shongjun{at}pennmedicine.upenn.edu), Juan Carlos de a Torre (juanct{at}scripps.edu) and Guo-li Ming (gming{at}pennmedicine.upenn.edu)
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Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism
Fadi Jacob, Sarshan R. Pather, Wei-Kai Huang, Samuel Zheng Hao Wong, Haowen Zhou, Feng Zhang, Beatrice Cubitt, Catherine Z. Chen, Miao Xu, Manisha Pradhan, Daniel Y. Zhang, Wei Zheng, Anne G. Bang, Hongjun Song, Juan Carlos de a Torre, Guo-li Ming
bioRxiv 2020.07.28.225151; doi: https://doi.org/10.1101/2020.07.28.225151
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Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism
Fadi Jacob, Sarshan R. Pather, Wei-Kai Huang, Samuel Zheng Hao Wong, Haowen Zhou, Feng Zhang, Beatrice Cubitt, Catherine Z. Chen, Miao Xu, Manisha Pradhan, Daniel Y. Zhang, Wei Zheng, Anne G. Bang, Hongjun Song, Juan Carlos de a Torre, Guo-li Ming
bioRxiv 2020.07.28.225151; doi: https://doi.org/10.1101/2020.07.28.225151

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