Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system

Alison Moss, Shaina Robbins, Sirisha Achanta, Lakshmi Kuttippurathu, Scott Turick, Sean Nieves, View ORCID ProfilePeter Hanna, Elizabeth H. Smith, View ORCID ProfileDonald B. Hoover, Jin Chen, Zixi (Jack) Cheng, Jeffrey L. Ardell, View ORCID ProfileKalyanam Shivkumar, View ORCID ProfileJames S. Schwaber, View ORCID ProfileRajanikanth Vadigepalli
doi: https://doi.org/10.1101/2020.07.29.227090
Alison Moss
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shaina Robbins
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sirisha Achanta
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lakshmi Kuttippurathu
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Scott Turick
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sean Nieves
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Hanna
2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center and Neurocardiology Research Program of Excellence, Department of Medicine, UCLA, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Peter Hanna
Elizabeth H. Smith
3Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Donald B. Hoover
3Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Donald B. Hoover
Jin Chen
4Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zixi (Jack) Cheng
4Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeffrey L. Ardell
2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center and Neurocardiology Research Program of Excellence, Department of Medicine, UCLA, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kalyanam Shivkumar
2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center and Neurocardiology Research Program of Excellence, Department of Medicine, UCLA, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kalyanam Shivkumar
  • For correspondence: kshivkumar@mednet.ucla.edu james.schwaber@jefferson.edu rajanikanth.vadigepalli@jefferson.edu
James S. Schwaber
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for James S. Schwaber
  • For correspondence: kshivkumar@mednet.ucla.edu james.schwaber@jefferson.edu rajanikanth.vadigepalli@jefferson.edu
Rajanikanth Vadigepalli
1Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rajanikanth Vadigepalli
  • For correspondence: kshivkumar@mednet.ucla.edu james.schwaber@jefferson.edu rajanikanth.vadigepalli@jefferson.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion - the right atrial ganglionic plexus (RAGP) that is a critical mediator of vagal control of the sinoatrial node (SAN) activity. We developed a 3D representation of RAGP with extensive mapping of neurons and used neuronal tracing to identify the spatial distribution of the subset of neurons that project to the SAN. RNAseq of laser capture microdissected neurons revealed a distinct composition of RAGP neurons compared to CNS neuronal subtypes. High-throughput qPCR of hundreds of laser capture microdissected single neurons led to a surprising finding that cholinergic and catecholaminergic neuronal markers Th and Chat were correlated, suggesting multipotential phenotypes that can drive neuroplasticity within RAGP. Interestingly, no single gene or module was an exclusive marker of RAGP neuronal connectivity to SAN. Neuropeptide-receptor coexpression analysis revealed a combinatorial paracrine neuromodulatory network within RAGP, informing follow-on studies on the vagal control of RAGP to regulate cardiac function in health and disease.

Competing Interest Statement

University of California, Los Angeles has patents developed by KS and JLA relating to cardiac neural diagnostics and therapeutics. KS and JLA are co-founders of NeuCures, Inc. All the remaining authors declare no competing interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted July 30, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system
Alison Moss, Shaina Robbins, Sirisha Achanta, Lakshmi Kuttippurathu, Scott Turick, Sean Nieves, Peter Hanna, Elizabeth H. Smith, Donald B. Hoover, Jin Chen, Zixi (Jack) Cheng, Jeffrey L. Ardell, Kalyanam Shivkumar, James S. Schwaber, Rajanikanth Vadigepalli
bioRxiv 2020.07.29.227090; doi: https://doi.org/10.1101/2020.07.29.227090
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A spatially-tracked single cell transcriptomics map of neuronal networks in the intrinsic cardiac nervous system
Alison Moss, Shaina Robbins, Sirisha Achanta, Lakshmi Kuttippurathu, Scott Turick, Sean Nieves, Peter Hanna, Elizabeth H. Smith, Donald B. Hoover, Jin Chen, Zixi (Jack) Cheng, Jeffrey L. Ardell, Kalyanam Shivkumar, James S. Schwaber, Rajanikanth Vadigepalli
bioRxiv 2020.07.29.227090; doi: https://doi.org/10.1101/2020.07.29.227090

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3607)
  • Biochemistry (7581)
  • Bioengineering (5529)
  • Bioinformatics (20809)
  • Biophysics (10338)
  • Cancer Biology (7988)
  • Cell Biology (11647)
  • Clinical Trials (138)
  • Developmental Biology (6611)
  • Ecology (10217)
  • Epidemiology (2065)
  • Evolutionary Biology (13630)
  • Genetics (9550)
  • Genomics (12854)
  • Immunology (7925)
  • Microbiology (19555)
  • Molecular Biology (7668)
  • Neuroscience (42147)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2203)
  • Physiology (3269)
  • Plant Biology (7051)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1952)
  • Systems Biology (5429)
  • Zoology (1119)