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3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system

Clara Leung, Shaina Robbins, Alison Moss, Maci Heal, Mahyar Osanlouy, Richard Christie, Navid Farahani, Corey Monteith, Jin Chen, View ORCID ProfilePeter Hunter, View ORCID ProfileSusan Tappan, View ORCID ProfileRajanikanth Vadigepalli, Zixi (Jack) Cheng, View ORCID ProfileJames S. Schwaber
doi: https://doi.org/10.1101/2020.07.29.227538
Clara Leung
1Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA
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Shaina Robbins
2Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
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Alison Moss
2Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
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Maci Heal
3MBF Bioscience, Williston, VT, USA
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Mahyar Osanlouy
4Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand
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Richard Christie
4Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand
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Navid Farahani
5Strateos, San Francisco, CA, USA
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Corey Monteith
5Strateos, San Francisco, CA, USA
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Jin Chen
1Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA
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Peter Hunter
4Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand
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Susan Tappan
3MBF Bioscience, Williston, VT, USA
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  • ORCID record for Susan Tappan
Rajanikanth Vadigepalli
2Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
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  • ORCID record for Rajanikanth Vadigepalli
  • For correspondence: rajanikanth.vadigepalli@jefferson.edu zixj.cheng@ucf.edu james.schwaber@jefferson.edu
Zixi (Jack) Cheng
1Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA
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  • For correspondence: rajanikanth.vadigepalli@jefferson.edu zixj.cheng@ucf.edu james.schwaber@jefferson.edu
James S. Schwaber
2Daniel Baugh Institute of Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA
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  • For correspondence: rajanikanth.vadigepalli@jefferson.edu zixj.cheng@ucf.edu james.schwaber@jefferson.edu
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Summary

We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across 4 male and 3 female hearts. We find the ICNS has a reliable structural organizational plan across individuals that may provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and using data-driven clustering. The pattern, distribution and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan in which distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density and slightly reduced distribution, but with the same localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for the standardization of common anatomical planes and providing the path where multiple experimental results and data types can be integrated and compared.

Competing Interest Statement

M.H. and S.T. are paid employees at MBF Bioscience and are funded under the OT3OD025349, to create multi-scale, multi-organ, and multi-species SPARC map management as a part of the SPARC Data Portal. Strateos is a commercial entity, and the authors affiliated with them are company employees. The remaining authors declare no competing interests.

Footnotes

  • Added a section on Limitations of the Study Reorganized the Methods Reformatted References and Figures

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted November 10, 2020.
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3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system
Clara Leung, Shaina Robbins, Alison Moss, Maci Heal, Mahyar Osanlouy, Richard Christie, Navid Farahani, Corey Monteith, Jin Chen, Peter Hunter, Susan Tappan, Rajanikanth Vadigepalli, Zixi (Jack) Cheng, James S. Schwaber
bioRxiv 2020.07.29.227538; doi: https://doi.org/10.1101/2020.07.29.227538
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3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system
Clara Leung, Shaina Robbins, Alison Moss, Maci Heal, Mahyar Osanlouy, Richard Christie, Navid Farahani, Corey Monteith, Jin Chen, Peter Hunter, Susan Tappan, Rajanikanth Vadigepalli, Zixi (Jack) Cheng, James S. Schwaber
bioRxiv 2020.07.29.227538; doi: https://doi.org/10.1101/2020.07.29.227538

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