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New tyrosinases with a broad spectrum of action against contaminants of emerging concern: Insights from in silico analyses

View ORCID ProfileMarcus V. X. Senra, View ORCID ProfileAna Lúcia Fonseca
doi: https://doi.org/10.1101/2020.07.30.225821
Marcus V. X. Senra
Instituto de Recursos Naturais, Universidade Federal de Itajubá, 37500-903, Itajubá, Minas Gerais – Brazil
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  • For correspondence: msenra@unifei.edu.br
Ana Lúcia Fonseca
Instituto de Recursos Naturais, Universidade Federal de Itajubá, 37500-903, Itajubá, Minas Gerais – Brazil
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Abstract

Tyrosinases (EC 1.14.18.1) are type-3 copper metalloenzymes with strong oxidative capacities and low allosteric selectivity to phenolic and non-phenolic aromatic compounds that have been used as biosensors and biocatalysts to mitigate the impacts of environmental contaminants over aquatic ecosystems. However, the widespread use of these polyphenol oxidases is limited by elevated production costs and restricted knowledge on their spectrum of action. Here, six tyrosinase homologs were identified and characterized from the genomes of 4 widespread freshwater ciliates using bioinformatics. Binding energies between 3D models of these homologs and ~1000 contaminants of emerging concern (CECs), including fine chemicals, pharmaceuticals, personal care products, illicit drugs, natural toxins, and pesticides were estimated through virtual screening, suggesting their spectrum of action and potential uses in environmental biotechnology might be considerably broader than previously thought. Moreover, considering that many ciliates, including those caring tyrosinase genes within their genomes are fast-growing unicellular microeukaryotes that can be efficiently culturable at large-scales under in vitro conditions, should be regarded as potential low-cost sources for the production of relevant biotechnological molecules.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 31, 2020.
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New tyrosinases with a broad spectrum of action against contaminants of emerging concern: Insights from in silico analyses
Marcus V. X. Senra, Ana Lúcia Fonseca
bioRxiv 2020.07.30.225821; doi: https://doi.org/10.1101/2020.07.30.225821
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New tyrosinases with a broad spectrum of action against contaminants of emerging concern: Insights from in silico analyses
Marcus V. X. Senra, Ana Lúcia Fonseca
bioRxiv 2020.07.30.225821; doi: https://doi.org/10.1101/2020.07.30.225821

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