Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Integrated tapered fibertrode for simultaneous control and readout of neural activity over small brain volumes with reduced light-induced artefacts

View ORCID ProfileB. Spagnolo, R.T. Peixoto, L. Sileo, M. Pisanello, F. Pisano, J.A. Assad, B.L. Sabatini, M. De Vittorio, F. Pisanello
doi: https://doi.org/10.1101/2020.07.31.226795
B. Spagnolo
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for B. Spagnolo
  • For correspondence: barbara.spagnolo@iit.it ferruccio.pisanello@iit.it massimo.devittorio@iit.it
R.T. Peixoto
3Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, (USA)
4Department of Psychiatry, University of Pittsburgh, 450 Technology Dr, Pittsburgh, PA 15219, (USA)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L. Sileo
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Pisanello
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. Pisano
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J.A. Assad
3Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, (USA)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B.L. Sabatini
3Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, (USA)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. De Vittorio
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
2Dipartimento di Ingegneria dell’Innovazione, Università del Salento, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: barbara.spagnolo@iit.it ferruccio.pisanello@iit.it massimo.devittorio@iit.it
F. Pisanello
1Istituto Italiano di Tecnologia, CBN, Arnesano, 73010 Lecce (Italy)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: barbara.spagnolo@iit.it ferruccio.pisanello@iit.it massimo.devittorio@iit.it
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Recognizing the neural patterns underlying different brain functions is essential to achieve a more comprehensive view on how small sets of neurons organize in complex 3D networks to determine different behaviours. In this framework optogenetic techniques have been successfully proven as a powerful tool to control brain functions achieving millisecond temporal resolution and cell-type specificity, by combining the use of light-gated opsins and ad-hoc light delivery optoelectronic devices. However, targeting small brain volumes with simultaneous electrical recording results in the introduction of photoelectric artefacts, in particular when light emission and recoding sites are very close one to each other. In this work we take advantage of the photonic properties of tapered fibers to present a fully integrated fibertrode to target small brain volumes with abated photoelectric noise. The device hosts a light emitting window just below a recording pad, and exploits the angled light emission from the window to achieve simultaneous activation and electrical readout of small groups of cells with no photoelectric artifacts in vivo. Despite the highly non-planar surface of the fiber taper, window’s size, shape and electrode’s impedance can be modulated by controlling the fabrication parameters during focused ion beam milling and deposition, thus resulting in a versatile, integrated and customizable optogenetic tool for neurobiology studies in closed-loop configuration over small brain volumes.

Competing Interest Statement

LS, BLS, MDV, and F. Pisanello are founders and hold private equity in Optogenix, a company that develops, produces and sells technologies to deliver light into the brain. Tapered fibers commercially available from Optogenix were used as tools in the research.

Footnotes

  • ↵† co-last authors

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted August 03, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Integrated tapered fibertrode for simultaneous control and readout of neural activity over small brain volumes with reduced light-induced artefacts
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Integrated tapered fibertrode for simultaneous control and readout of neural activity over small brain volumes with reduced light-induced artefacts
B. Spagnolo, R.T. Peixoto, L. Sileo, M. Pisanello, F. Pisano, J.A. Assad, B.L. Sabatini, M. De Vittorio, F. Pisanello
bioRxiv 2020.07.31.226795; doi: https://doi.org/10.1101/2020.07.31.226795
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Integrated tapered fibertrode for simultaneous control and readout of neural activity over small brain volumes with reduced light-induced artefacts
B. Spagnolo, R.T. Peixoto, L. Sileo, M. Pisanello, F. Pisano, J.A. Assad, B.L. Sabatini, M. De Vittorio, F. Pisanello
bioRxiv 2020.07.31.226795; doi: https://doi.org/10.1101/2020.07.31.226795

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4117)
  • Biochemistry (8820)
  • Bioengineering (6523)
  • Bioinformatics (23470)
  • Biophysics (11798)
  • Cancer Biology (9216)
  • Cell Biology (13327)
  • Clinical Trials (138)
  • Developmental Biology (7440)
  • Ecology (11417)
  • Epidemiology (2066)
  • Evolutionary Biology (15160)
  • Genetics (10442)
  • Genomics (14051)
  • Immunology (9176)
  • Microbiology (22170)
  • Molecular Biology (8817)
  • Neuroscience (47600)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3733)
  • Plant Biology (8084)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1254)