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Synaptic contributions to cochlear outer hair cell Ca2+ homeostasis

Marcelo J. Moglie, Diego L. Wengier, A. Belén Elgoyhen, Juan D. Goutman
doi: https://doi.org/10.1101/2020.08.02.233205
Marcelo J. Moglie
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI), C. A. Buenos Aires (1428) Argentina
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Diego L. Wengier
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI), C. A. Buenos Aires (1428) Argentina
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A. Belén Elgoyhen
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI), C. A. Buenos Aires (1428) Argentina
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Juan D. Goutman
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI), C. A. Buenos Aires (1428) Argentina
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  • For correspondence: jgoutman@gmail.com
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Abstract

For normal cochlear function, outer hair cells (OHCs) require a precise regulation of intracellular Ca2+ levels. Influx of Ca2+ occurs both at the stereocillia tips and through the basolateral membrane. In this latter compartment, two different origins for Ca2+ influx have been poorly explored: voltage-gated Ca2+ channels (VGCC) at synapses with type II afferent neurons, and α9α10 cholinergic nicotinic receptors at synapses with medio-olivochlear complex (MOC) neurons. Using functional imaging, we report that these two Ca2+ entry sites are closely positioned, but present different regulation mechanisms. Ca2+ spread from MOC synapses is contained by cisternal Ca2+-ATPases. Considered a weak drive for transmitter release, we found that VGCC Ca2+ signals are larger than expected and can be potentiated by ryanodine receptors. Finally, we showed that sorcin, a highly expressed gene product in OHCs with reported Ca2+ control function in cardiomyocytes, regulates basal Ca2+ levels and MOC synaptic activity in OHCs.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 02, 2020.
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Synaptic contributions to cochlear outer hair cell Ca2+ homeostasis
Marcelo J. Moglie, Diego L. Wengier, A. Belén Elgoyhen, Juan D. Goutman
bioRxiv 2020.08.02.233205; doi: https://doi.org/10.1101/2020.08.02.233205
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Synaptic contributions to cochlear outer hair cell Ca2+ homeostasis
Marcelo J. Moglie, Diego L. Wengier, A. Belén Elgoyhen, Juan D. Goutman
bioRxiv 2020.08.02.233205; doi: https://doi.org/10.1101/2020.08.02.233205

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