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Seed hemicelluloses tailor mucilage properties and salt tolerance

View ORCID ProfileBo Yang, Florian Hofmann, View ORCID ProfileBjörn Usadel, View ORCID ProfileCătălin Voiniciuc
doi: https://doi.org/10.1101/2020.08.03.234708
Bo Yang
1Institute for Botany and Molecular Genetics (IBMG), BioSC, RWTH Aachen University, 52074 Aachen, Germany
2Independent Junior Research Group–Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
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Florian Hofmann
1Institute for Botany and Molecular Genetics (IBMG), BioSC, RWTH Aachen University, 52074 Aachen, Germany
3Institute for Bio- and Geosciences (IBG-2: Plant Sciences), Forschungszentrum Jülich, 52425 Jülich, Germany
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Björn Usadel
1Institute for Botany and Molecular Genetics (IBMG), BioSC, RWTH Aachen University, 52074 Aachen, Germany
3Institute for Bio- and Geosciences (IBG-2: Plant Sciences), Forschungszentrum Jülich, 52425 Jülich, Germany
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Cătălin Voiniciuc
1Institute for Botany and Molecular Genetics (IBMG), BioSC, RWTH Aachen University, 52074 Aachen, Germany
2Independent Junior Research Group–Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
3Institute for Bio- and Geosciences (IBG-2: Plant Sciences), Forschungszentrum Jülich, 52425 Jülich, Germany
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  • For correspondence: catalin.voiniciuc@ipb-halle.de
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Summary

  • While Arabidopsis seed coat epidermal cells have become an excellent genetic system to study the biosynthesis and structural roles of various cell wall polymers, the physiological function of the secreted mucilaginous polysaccharides remains ambiguous. Seed mucilage is shaped by two distinct classes of highly substituted hemicelluloses along with cellulose and structural proteins, but their interplay has not been explored.

  • We deciphered the functions of four distinct classes of cell wall polymers by generating a series of double mutants with defects in heteromannan, xylan, cellulose, or the arabinogalactan protein SALT-OVERLY SENSITIVE 5 (SOS5), and evaluating their impact on mucilage architecture and on seed germination during salt stress.

  • We discovered that muci10 seeds, lacking heteromannan branches, had elevated tolerance to salt stress, while heteromannan elongation mutants exhibited reduced germination in CaCl2. In contrast, xylan made by MUCILAGE-RELATED21 (MUCI21) was found to be required for the adherence of mucilage pectin to microfibrils made by CELLULOSE SYNTHASE5 (CESA5) as well as to a SOS5-mediated network.

  • Our results indicate that the substitution of xylan and glucomannan in seeds can fine-tune mucilage adherence and salt tolerance, respectively. The study of germinating seeds can thus provide insights into the synthesis, modification and function of complex glycans.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • E-Mails, bo.yang{at}ipb-halle.de, f.hofmann35{at}gmail.com, b.usadel{at}fz-juelich.de, catalin.voiniciuc{at}ipb-halle.de

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 01, 2020.
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Seed hemicelluloses tailor mucilage properties and salt tolerance
Bo Yang, Florian Hofmann, Björn Usadel, Cătălin Voiniciuc
bioRxiv 2020.08.03.234708; doi: https://doi.org/10.1101/2020.08.03.234708
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Seed hemicelluloses tailor mucilage properties and salt tolerance
Bo Yang, Florian Hofmann, Björn Usadel, Cătălin Voiniciuc
bioRxiv 2020.08.03.234708; doi: https://doi.org/10.1101/2020.08.03.234708

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