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Hypertonic saline solution inhibits SARS-CoV-2 in vitro assay

View ORCID ProfileRafael R. G. Machado, View ORCID ProfileTalita Glaser, View ORCID ProfileDanielle B. Araujo, Lyvia Lintzmaier Petiz, Danielle B. L. Oliveira, Giuliana S. Durigon, Alessandra L. Leal, João Renato R. Pinho, View ORCID ProfileLuis Carlos S. Ferreira, View ORCID ProfileHenning Ulrich, View ORCID ProfileEdison L. Durigon, View ORCID ProfileCristiane R. Guzzo
doi: https://doi.org/10.1101/2020.08.04.235549
Rafael R. G. Machado
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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Talita Glaser
2Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
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Danielle B. Araujo
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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Lyvia Lintzmaier Petiz
2Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
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Danielle B. L. Oliveira
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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Giuliana S. Durigon
3Medical School Clinic Hospital, University of São Paulo, São Paulo, Brazil
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Alessandra L. Leal
4Gaspar Vianna Clinic Hospital Foundation, Belém, Brazil
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João Renato R. Pinho
5Hospital Israelita Albert Einstein, São Paulo, Brazil; LIM-03, Central Laboratories Division, Clinics Hospital, São Paulo School of Medicine, University of São Paulo, São Paulo, Brazil; LIM-07, Institute of Tropical Medicine, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, Brazil
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Luis Carlos S. Ferreira
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
6Scientific Platform Pasteur USP, São Paulo, Brazil
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Henning Ulrich
2Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil
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  • For correspondence: eldurigo@usp.br crisguzzo@usp.br henning@iq.usp.br
Edison L. Durigon
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
6Scientific Platform Pasteur USP, São Paulo, Brazil
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  • For correspondence: eldurigo@usp.br crisguzzo@usp.br henning@iq.usp.br
Cristiane R. Guzzo
1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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  • ORCID record for Cristiane R. Guzzo
  • For correspondence: eldurigo@usp.br crisguzzo@usp.br henning@iq.usp.br
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Abstract

We are facing an unprecedented global health crisis caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At this date more than 680 thousand people have died due to coronavirus disease 2019 (COVID-19). Unfortunately, until now no effective treatment to combat the virus and vaccine are available. We performed experiments to test if hypertonic saline solution is able to inhibit virus replication in vitro. Our data shows that 260 mM NaCl (1.5%) inhibits 100% SARS-CoV-2 replication in Vero cells. Furthermore, our results suggest that the virus replication inhibition is due to an intracellular mechanism and not due to the dissociation between spike SARS-CoV-2 protein and its human receptor angiotensin-converting enzyme 2 interaction. NaCl depolarizes the plasma membrane supposedly associated with the inhibition of the SARS-CoV-2 life cycle. This observation could lead to simple, safe and low cost interventions at various stages of COVID-19 treatment, improving the prognosis of infected patients, thereby mitigating the social and economic costs of the pandemic.

Competing Interest Statement

The authors have declared no competing interest.

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Posted August 04, 2020.
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Hypertonic saline solution inhibits SARS-CoV-2 in vitro assay
Rafael R. G. Machado, Talita Glaser, Danielle B. Araujo, Lyvia Lintzmaier Petiz, Danielle B. L. Oliveira, Giuliana S. Durigon, Alessandra L. Leal, João Renato R. Pinho, Luis Carlos S. Ferreira, Henning Ulrich, Edison L. Durigon, Cristiane R. Guzzo
bioRxiv 2020.08.04.235549; doi: https://doi.org/10.1101/2020.08.04.235549
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Hypertonic saline solution inhibits SARS-CoV-2 in vitro assay
Rafael R. G. Machado, Talita Glaser, Danielle B. Araujo, Lyvia Lintzmaier Petiz, Danielle B. L. Oliveira, Giuliana S. Durigon, Alessandra L. Leal, João Renato R. Pinho, Luis Carlos S. Ferreira, Henning Ulrich, Edison L. Durigon, Cristiane R. Guzzo
bioRxiv 2020.08.04.235549; doi: https://doi.org/10.1101/2020.08.04.235549

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