Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Kinetics and Fidelity of Psychrophilic DNA Polymerases

View ORCID ProfileYuan Xue, View ORCID ProfileIdo Braslavsky, View ORCID ProfileStephen R. Quake
doi: https://doi.org/10.1101/2020.08.04.236919
Yuan Xue
1Department of Bioengineering, Stanford University, Stanford, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yuan Xue
Ido Braslavsky
2The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem, Rehovot 7610001, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ido Braslavsky
Stephen R. Quake
1Department of Bioengineering, Stanford University, Stanford, CA, USA
3Department of Applied Physics, Stanford University, Stanford, CA, USA
4Chan Zuckerberg Biohub, Mission Bay, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stephen R. Quake
  • For correspondence: steve@quake-lab.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The discovery of extremophiles has enabled development of groundbreaking biotechnology. While most of the extremophile research is focused on thermophiles, organisms that adapt to living in cold temperature, known as psychrophiles, remain under-studied. We expressed and purified DNA polymerases PIPI and PIPB from Psychromonas ingrahamii, a psychrophile that can grow below water’s freezing temperature. We demonstrate that they have in vitro DNA replication activity at temperatures as low as −19°C. To our knowledge, this is the coolest DNA polymerization reaction ever carried out in a laboratory. In exploring the behavior of a variety of polymerases as a function of temperature, we found that reaction temperature substantially increases substitution and deletion error rates of both psychrophilic and mesophilic DNA polymerases. Motif analysis further reveals that the substitution error profiles cluster according to phylogenetic similarity of polymerases. Our results provide a useful reference for how reaction temperature, a crucial parameter of biochemistry, can affect the fidelity of DNA polymerases adapted to a wide range of environment.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted August 05, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Kinetics and Fidelity of Psychrophilic DNA Polymerases
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Kinetics and Fidelity of Psychrophilic DNA Polymerases
Yuan Xue, Ido Braslavsky, Stephen R. Quake
bioRxiv 2020.08.04.236919; doi: https://doi.org/10.1101/2020.08.04.236919
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Kinetics and Fidelity of Psychrophilic DNA Polymerases
Yuan Xue, Ido Braslavsky, Stephen R. Quake
bioRxiv 2020.08.04.236919; doi: https://doi.org/10.1101/2020.08.04.236919

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (3512)
  • Biochemistry (7352)
  • Bioengineering (5329)
  • Bioinformatics (20277)
  • Biophysics (10026)
  • Cancer Biology (7749)
  • Cell Biology (11319)
  • Clinical Trials (138)
  • Developmental Biology (6440)
  • Ecology (9958)
  • Epidemiology (2065)
  • Evolutionary Biology (13336)
  • Genetics (9362)
  • Genomics (12592)
  • Immunology (7714)
  • Microbiology (19045)
  • Molecular Biology (7447)
  • Neuroscience (41063)
  • Paleontology (300)
  • Pathology (1231)
  • Pharmacology and Toxicology (2139)
  • Physiology (3164)
  • Plant Biology (6866)
  • Scientific Communication and Education (1274)
  • Synthetic Biology (1898)
  • Systems Biology (5318)
  • Zoology (1089)