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Heat-treated virus inactivation rate depends strongly on treatment procedure: illustration with SARS-CoV-2

View ORCID ProfileAmandine Gamble, View ORCID ProfileRobert J. Fischer, View ORCID ProfileDylan H. Morris, View ORCID ProfileKwe Claude Yinda, View ORCID ProfileVincent J. Munster, View ORCID ProfileJames O. Lloyd-Smith
doi: https://doi.org/10.1101/2020.08.10.242206
Amandine Gamble
1Department of Ecology & Evolutionary Biology, University of California, Los Angeles, CA, USA
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  • For correspondence: amandine.gamble@gmail.com
Robert J. Fischer
2Laboratory of Virology, National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA
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Dylan H. Morris
3Department of Ecology & Evolutionary Biology, Princeton University, NJ, USA
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Kwe Claude Yinda
2Laboratory of Virology, National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA
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Vincent J. Munster
2Laboratory of Virology, National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA
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James O. Lloyd-Smith
1Department of Ecology & Evolutionary Biology, University of California, Los Angeles, CA, USA
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Abstract

Decontamination helps limit environmental transmission of infectious agents. It is required for the safe re-use of contaminated medical, laboratory and personal protective equipment, and for the safe handling of biological samples. Heat treatment is a common decontamination method, notably used for viruses. We show that for liquid specimens (here, solution of SARS-CoV-2 in cell culture medium), virus inactivation rate under heat treatment at 70°C can vary by almost two orders of magnitude depending on the treatment procedure, from a half-life of 0.86 min (95% credible interval: [0.09, 1.77]) in closed vials in a heat block to 37.00 min ([12.65, 869.82]) in uncovered plates in a dry oven. These findings suggest a critical role of evaporation in virus inactivation via dry heat. Placing samples in open or uncovered containers may dramatically reduce the speed and efficacy of heat treatment for virus inactivation. Given these findings, we reviewed the literature temperature-dependent coronavirus stability and found that specimen containers, and whether they are closed, covered, or uncovered, are rarely reported in the scientific literature. Heat-treatment procedures must be fully specified when reporting experimental studies to facilitate result interpretation and reproducibility, and must be carefully considered when developing decontamination guidelines.

Importance Heat is a powerful weapon against most infectious agents. It is widely used for decontamination of medical, laboratory and personal protective equipment, and for biological samples. There are many methods of heat treatment, and methodological details can affect speed and efficacy of decontamination. We applied four different heat-treatment procedures to liquid specimens containing SARS-CoV-2. Our results show that the container used to store specimens during decontamination can substantially affect inactivation rate: for a given initial level of contamination, decontamination time can vary from a few minutes in closed vials to several hours in uncovered plates. Reviewing the literature, we found that container choices and heat treatment methods are only rarely reported explicitly in methods sections. Our study shows that careful consideration of heat-treatment procedure — in particular the choice of specimen container, and whether it is covered — can make results more consistent across studies, improve decontamination practice, and provide insight into the mechanisms of virus inactivation.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • The manuscript was re-organized to fit editorial constrains of the targeted journal. In particular, the Results and Discussion sections were merged. Other minor edits were made with the aim of clarifying the manuscript. The content of the study is unchanged.

  • https://github.com/dylanhmorris/heat-inactivation

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 07, 2021.
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Heat-treated virus inactivation rate depends strongly on treatment procedure: illustration with SARS-CoV-2
Amandine Gamble, Robert J. Fischer, Dylan H. Morris, Kwe Claude Yinda, Vincent J. Munster, James O. Lloyd-Smith
bioRxiv 2020.08.10.242206; doi: https://doi.org/10.1101/2020.08.10.242206
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Heat-treated virus inactivation rate depends strongly on treatment procedure: illustration with SARS-CoV-2
Amandine Gamble, Robert J. Fischer, Dylan H. Morris, Kwe Claude Yinda, Vincent J. Munster, James O. Lloyd-Smith
bioRxiv 2020.08.10.242206; doi: https://doi.org/10.1101/2020.08.10.242206

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