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Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae

Jonna Heldrich, Carolyn R. Milano, Tovah E. Markowitz, Sarah N. Ur, View ORCID ProfileLuis A. Vale-Silva, Kevin D. Corbett, View ORCID ProfileAndreas Hochwagen
doi: https://doi.org/10.1101/2020.08.11.247122
Jonna Heldrich
1Department of Biology, New York University, New York, NY 10003, USA
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Carolyn R. Milano
1Department of Biology, New York University, New York, NY 10003, USA
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Tovah E. Markowitz
1Department of Biology, New York University, New York, NY 10003, USA
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Sarah N. Ur
2Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
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Luis A. Vale-Silva
1Department of Biology, New York University, New York, NY 10003, USA
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Kevin D. Corbett
2Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
3Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
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Andreas Hochwagen
1Department of Biology, New York University, New York, NY 10003, USA
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  • ORCID record for Andreas Hochwagen
  • For correspondence: andi@nyu.edu
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ABSTRACT

Successful meiotic recombination, and thus fertility, depends on conserved axis proteins that organize chromosomes into arrays of anchored chromatin loops and provide a protected environment for DNA exchange. Here, we show that the stereotypic chromosomal distribution of axis proteins in S. cerevisiae is the additive result of two independent pathways: a cohesin-dependent pathway, which was previously identified and mediates focal enrichment of axis proteins at gene ends, and a parallel cohesin-independent pathway that recruits axis proteins to broad genomic islands with high gene density. These islands exhibit elevated markers of crossover recombination as well as increased nucleosome density, which we show is a direct consequence of the underlying DNA sequence. A predicted PHD domain in the center of the axis factor Hop1 specifically mediates cohesin-independent axis recruitment. Intriguingly, other chromosome organizers, including cohesin, condensin, and topoisomerases, are differentially depleted from the same regions even in non-meiotic cells, indicating that these DNA sequence-defined chromatin islands exert a general influence on the patterning of chromosome structure.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted April 04, 2022.
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Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae
Jonna Heldrich, Carolyn R. Milano, Tovah E. Markowitz, Sarah N. Ur, Luis A. Vale-Silva, Kevin D. Corbett, Andreas Hochwagen
bioRxiv 2020.08.11.247122; doi: https://doi.org/10.1101/2020.08.11.247122
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Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae
Jonna Heldrich, Carolyn R. Milano, Tovah E. Markowitz, Sarah N. Ur, Luis A. Vale-Silva, Kevin D. Corbett, Andreas Hochwagen
bioRxiv 2020.08.11.247122; doi: https://doi.org/10.1101/2020.08.11.247122

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